A comparative study of staining character of mycobacteria
Mahapatra, B. (1967) A comparative study of staining character of mycobacteria. Indian Journal of Tuberculosis, 15 (1). pp. 17-18.
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150 samples of tuberculous materials comprising of bacterial culture, sputa, urine, effusion fluid, gastric juice, skin smear and microsections were stained both with Ziehl- Neelsen and Pottz’s rapid and cold method and were examined for acid fast bacilli. Although number of positive samples show- ing the A.F.B. were almost same, there was definite difference in the staining intensity of the bacilli. The possible cause for faint stain- ing of the bacilli in the cold method has been discussed. It is felt that the cold method of staining as advocated by Pottz and his associates can not be considered as a proper substitute for Z.N. method.
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