Laboratory generated artifacts in plasma amino acid quantitation

Nanjunda, Ananth (2002) Laboratory generated artifacts in plasma amino acid quantitation. Online Journal of Health and Allied Sciences, 1 (3). ISSN 0972-5997

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The pace of physicians’ involvement in amino acid metabolism has been enormous in the last five decades. With further development of technology to identify and quantitate upto picomoles of amino acids, their metabolites and related peptides, diagnosis and effective medical intervention in cases of inherited metabolic disorders have been well within the reach of the clinician. Automatic amino acid analyzers have become an essential part of major medical and research centers around the world. The technology has come indeed as a boon to physicians who in particular deal with inherited defects of amino acid metabolism. However, the technology comes with the risk of major deviations from the actual results when a few minor variations are not looked into. Trivial variations in basic steps of obtaining the sample, the choice of anticoagulant, hemolysis etc. can cause significant variations in the resulting values, particularly while dealing with inherited defects of amino acid metabolism and their treatment/management. Effects of such factors are revisited here for the benefit of the modern day laboratory personnel.

EPrint Type:Article
Uncontrolled Keywords:Amino acid quantitation; Artifacts; Inherited metabolic disorders
Subjects:Amino Acids, Peptides, and Proteins > Amino Acids
Nutritional and Metabolic Diseases > Metabolic Diseases
Biochemical Phenomena, Metabolism, and Nutrition > Metabolism
-Journal Repositories > Online Journal of Health and Allied Sciences
Health Care Facilities, Manpower, and Services > Health Facilities
ID Code:1297
Deposited By:Dr Srinivas Kakkilaya
Deposited On:24 January 2006

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