Antimicrobial activity of Nigella sativa oil against multi-drug resistant Pseudomonas aerugenosa from clinical specimens
Salman, Mohd Tariq; Khan, Rahat Ali and Shukla, Indu (2005) Antimicrobial activity of Nigella sativa oil against multi-drug resistant Pseudomonas aerugenosa from clinical specimens. In: XIV Annual Conference of Indian Association of Pathologists & Microbiologists (UP Chapter) - UP-PATHMICON-2005, 23 Nov 2005, Aligarh, India.
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Objective: To study the effect of Nigella sativa essential oil against standard and multidrug resistant strains of Pseudomonas aerugenosa. Methods: N. sativa oil (Mohammedia products, Hyderabad) was tested in various dilutions by Disc Agar diffusion technique using impregnated filter paper discs on inoculated Muellar Hinton agar plates against P. aerugenosa (ATCC 27853 and NCTC 10662) and 22 clinical isolates of Pseudomonas obtained from pus, blood, ear discharge and conjunctival swab of patients attending J.N. Medical College Hospital, Aligarh during 2003 and 2004. Out of the clinical isolates taken, 3 were resistant to 2-4 antibiotics, 6 to 5-7 antibiotics, 8 to 8-10 antibiotics and 5 to 11-13 antibiotics. Resistance was highest for Ampicillin, Gentamicin, Ciprofloxacin, Amikacin, Ceftazidime, Cefotaxime, Ofloxacin and Ceftriaxone. Results: The oil inhibited P. aerugenosa ATCC 27853 and NCTC 10662 upto a dilution of 1:50 and 1:100 respectively. It was active upto 1:50 dilution against 1 strain resistant to 6 antibiotics, upto 1:10 dilution against 5 strains resistant to 2-11 antibiotics and in undiluted state against 6 strains resistant to 6-11 antibiotics. Conclusion: N. sativa oil is active against standard as well as multi-drug resistant strains of P. aerugenosa and may be used therapeutically in susceptible cases.
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