Antimicrobial activity of Nigella sativa oil against Staphylococcus aureus obtained from clinical specimens

Salman, Mohd Tariq; Khan, Rahat Ali and Shukla, Indu (2005) Antimicrobial activity of Nigella sativa oil against Staphylococcus aureus obtained from clinical specimens. In: 38th Annual Conference of Indial Pharmacological Society, 28- 30 Dec 2005, Chennai, India.

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Abstract

Objective: To study the effect of Nigella sativa essential oil on sensitive and multidrug resistant strains of S. aureus.Methods: Fifty-four strains of S. aureus were obtained from clinical specimens (pus, blood, conjunctival swab, cervical swab, urine, ear discharge, etc) from patients attending J.N. medical College Hospital, Aligarh during 2003 and 2004. Their sensitivities were tested to N. sativa oil (obtained from Mohammedia products, Hyderabad) in 1:1, 1:10 and 1:50 dilutions along with 20 other antibiotics by Disc Agar diffusion technique using impregnated filter paper discs on inoculated Muellar Hinton agar plates. Results: Out of 54 strains tested, 11 were resistant to 1-3 antibiotics, 16 to 4-6 antibiotics, 16 to 7-9 antibiotics, 3 to 10-12 antibiotics, 5 to 13-15 antibiotics and 1 to 16 antibiotics. Resistance was highest to Amikacin, followed by Tetracycline, Cotrimoxazole, Ciprofloxacin, Ampicillin, Ceftriaxone, Tobramycin, Gentamicin and Erythromycin. The oil was found to be active against 50 strains. It was active upto a dilution of ≤ 1:50 in 22 strains, upto 1:10 in 14 and only in undiluted state in 14 strains.Conclusion: N. sativa oil is active against sensitive as well multi drug resistant strains of S. aureus and may be used therapeutically in susceptible cases.

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