Study on the effect of green pit viper venom (Trimeresurus albolabris) on platelet morphology by flow cytometry

Soogarun, Suphan; Choewbamrungkiat, Montri; Wiwanitkit, Viroj; Suwansaksri, Jamsai; Nopanitaya, Waykin; Pradniwat, Paweena; Palasuwan, Attakorn and Chanprasert, Supantitra (2006) Study on the effect of green pit viper venom (Trimeresurus albolabris) on platelet morphology by flow cytometry. Online Journal Of Health And Allied Sciences, 5 (3). ISSN 0972-5997

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Abstract

The incidence of venomous snake bites, especially by green pit viper, has been increasing every year in Thailand. The bite of green pit viper causes bleeding because of thrombin–like property of the venom and it has been reported that the mean platelet volume decreases in those bitten by this snake. The objective of this study was to study the effect of green pit viper venom (Trimeresurus albolabris) on platelet volume (MPV), number of platelets and platelet morphology in vitro. The test was carried out by washing platelet in phosphate buffer pH 7.2 so as to get rid of fibrinogen, then the washed platelets were mixed with green pit viper venom. The mean platelet volume and number of platelets were determined by flow cytometry. The results showed that there was a decrease in the number of platelets (216±101 x 109/l and 78.1±43.4 x 109/l; P< 0.05) and also the MPV (8.9±1.2fl and 4.8±1.3fl, P< 0.05). The platelet size was smaller than normal, ranging from 1.1-1.2 micrometers. In conclusion, the green pit viper venom had a direct effect on platelet morphology, especially by decreasing platelet volume and numbers.

EPrint Type:Article
Uncontrolled Keywords:Green pit viper, Venom, Platelet, Snake bites, Trimeresurus alboloabris, Platelet Venom
Subjects:Diagnosis > Diagnostic Techniques and Procedures
Complex Mixtures > Venoms > Snake Venoms
Hematologic, Gastrointestinal, and Renal Agents > Hematologic Agents > Platelet Aggregation Inhibitors
-Journal Repositories > Online Journal of Health and Allied Sciences
Enzymes and Coenzymes > Enzymes > Hydrolases
Diagnosis > Laboratory Techniques and Procedures
Immunologic and Biological Factors > Biological Factors > Blood Coagulation Factors
ID Code:1958
Deposited By:Dr Srinivas Kakkilaya
Deposited On:22 December 2006

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