Effect of metC mutation on Salmonella gallinarum virulence and invasiveness in 1-day-old white leghorn chickens

Shah, D.H.; Shringi, Smriti; Desai, A.R.; Heo, E.J.; Park, J.H. and Chae, J.S. (2007) Effect of metC mutation on Salmonella gallinarum virulence and invasiveness in 1-day-old white leghorn chickens. Veterinary Microbiology, 119. pp. 352-357.

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Abstract

Salmonella enterica serotype Gallinarum (S. Gallinarum) is the causative agent of fowl typhoid (FT) in chickens. FT is a severe systemic disease of chickens causing heavy economic losses to the poultry industry through mortality, reduced egg production and culling of precious breeding stocks. In this study, a metC (encoding cystathionine β lyase) mutant was produced from a virulent strain of S. Gallinarum by Mini-Tn5 insertional inactivation. The mutant was significantly attenuated in virulence for 1-day-old White Leghorn chickens. Inactivation of metC resulted in 104-fold increase in the LD50 when compared with the wild type parent. The metC mutant showed an in vivo competitiveness defect in the challenged chickens and significantly lower (P <0.01) bacterial burden in the reticuloendothelial organs when compared with the wild-type parent. These results indicate that metC gene is important for virulence of S. Gallinarum in chickens.

EPrint Type:Article
Uncontrolled Keywords:Salmonella gallinarum, Chicken, metC, Virulence
Subjects:Animal Diseases > Salmonella Infections, Animal
Animal Diseases > Bird Diseases > Poultry Diseases
Bacterial Infections and Mycoses > Bacterial Infections > Gram-Negative Bacterial Infections
Immunologic and Biological Factors > Biological Factors > Virulence Factors
Animals > Chordata > Vertebrates > Birds > Poultry
Bacteria > Gram-Negative Bacteria
ID Code:1996
Deposited By:Dr. Devendra Shah
Deposited On:23 January 2007

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