Effect of metC mutation on Salmonella gallinarum virulence and invasiveness in 1-day-old white leghorn chickens
Shah, D.H.; Shringi, Smriti; Desai, A.R.; Heo, E.J.; Park, J.H. and Chae, J.S. (2007) Effect of metC mutation on Salmonella gallinarum virulence and invasiveness in 1-day-old white leghorn chickens. Veterinary Microbiology, 119. pp. 352-357.
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Salmonella enterica serotype Gallinarum (S. Gallinarum) is the causative agent of fowl typhoid (FT) in chickens. FT is a severe systemic disease of chickens causing heavy economic losses to the poultry industry through mortality, reduced egg production and culling of precious breeding stocks. In this study, a metC (encoding cystathionine β lyase) mutant was produced from a virulent strain of S. Gallinarum by Mini-Tn5 insertional inactivation. The mutant was significantly attenuated in virulence for 1-day-old White Leghorn chickens. Inactivation of metC resulted in 104-fold increase in the LD50 when compared with the wild type parent. The metC mutant showed an in vivo competitiveness defect in the challenged chickens and significantly lower (P <0.01) bacterial burden in the reticuloendothelial organs when compared with the wild-type parent. These results indicate that metC gene is important for virulence of S. Gallinarum in chickens.
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