Genetic differentiation of Indian camel (Camelus dromedarius) breeds using random oligonucleotide primers
Mehta, S.C.; Mishra, B.P. and Sahani, M.S. (2006) Genetic differentiation of Indian camel (Camelus dromedarius) breeds using random oligonucleotide primers. Animal Genetic Resources Information, 39. pp. 77-88. ISSN 1014-2339
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Camel population in India is facing a severe decline which demands that immediate steps are taken to ensure its conservation. Characterization is an integral part of the conservation program. The Polymerase Chain Reaction – Randomly Amplified Polymorphic DNA profile of unrelated camels of Bikaneri (29), Jaisalmeri (30) and Kachchhi (18) breeds were analyzed. Reproducible polymorphic bands with varying frequencies among the three breeds of camel were obtained with five oligonucleotide primers. A total of 75 bands were amplified, of which 27 (36%) were polymorphic. The probability of obtaining identical fingerprints was observed to be the lowest in primer GC-10 (5.7%) followed by OP-08 (8.7%), GT-10 (11.3%), G-2 (15.5% and G-1 (80%). Breed informative bands were amplified. The maximum genetic variability was observed in Bikaneri (0.800.05) followed by Kachchhi (0.840.06) and Jaisalmeri (0.870.05) breeds. The inter-breed genetic distance estimates indicated closer relationship in Bikaneri-Kachchhi (0.075) followed by Jaisalmeri-Kachchhi (0.106) and Bikaneri-Jaisalmeri (0.132) breeds. Similar genetic relationship was observed when the degree of population subdivision was measured between Bikaneri-Kachchhi (0.529), Jaisalmeri-Kachchhi (0.558) and Bikaneri-Jaisalmeri (0.566) breeds.
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