Genetic differentiation of Indian camel (Camelus dromedarius) breeds using random oligonucleotide primers

Mehta, S.C.; Mishra, B.P. and Sahani, M.S. (2006) Genetic differentiation of Indian camel (Camelus dromedarius) breeds using random oligonucleotide primers. Animal Genetic Resources Information, 39. pp. 77-88. ISSN 1014-2339

Full text available as:

PDF - Requires Adobe Acrobat Reader or other PDF viewer.
60 Kb

Abstract

Camel population in India is facing a severe decline which demands that immediate steps are taken to ensure its conservation. Characterization is an integral part of the conservation program. The Polymerase Chain Reaction – Randomly Amplified Polymorphic DNA profile of unrelated camels of Bikaneri (29), Jaisalmeri (30) and Kachchhi (18) breeds were analyzed. Reproducible polymorphic bands with varying frequencies among the three breeds of camel were obtained with five oligonucleotide primers. A total of 75 bands were amplified, of which 27 (36%) were polymorphic. The probability of obtaining identical fingerprints was observed to be the lowest in primer GC-10 (5.7%) followed by OP-08 (8.7%), GT-10 (11.3%), G-2 (15.5% and G-1 (80%). Breed informative bands were amplified. The maximum genetic variability was observed in Bikaneri (0.800.05) followed by Kachchhi (0.840.06) and Jaisalmeri (0.870.05) breeds. The inter-breed genetic distance estimates indicated closer relationship in Bikaneri-Kachchhi (0.075) followed by Jaisalmeri-Kachchhi (0.106) and Bikaneri-Jaisalmeri (0.132) breeds. Similar genetic relationship was observed when the degree of population subdivision was measured between Bikaneri-Kachchhi (0.529), Jaisalmeri-Kachchhi (0.558) and Bikaneri-Jaisalmeri (0.566) breeds.

EPrint Type:Article
Uncontrolled Keywords:Characterization, Camel, Oligonucleotide Primers, Breeding, Genetic Differentiation, DNA Profile, Polymerase Chain Reaction, Randomly Amplified Polymorphic DNA profile
Subjects:Investigative Techniques > Genetic Techniques
Population Characteristics > Population
Nucleic Acids, Nucleotides, and Nucleosides > Nucleic Acids > DNA
Genetic Phenomena > Variation (Genetics)
Genetic Processes > Breeding
Animals > Chordata > Vertebrates > Mammals > Artiodactyla > Ruminants > Camels
ID Code:2093
Deposited By:Dr S.C. Mehta
Deposited On:03 May 2007

Archive Staff Only: edit this record