Detection of infectious bursal disease virus (IBDV) from bursal tissue by RTPCR and its comparative efficacy with conventional precipitation assays

Makadiya, N.R.; Jhala, M.K.; Gaba, Amit; Joshi, C.G. and Rank, D.N. (2006) Detection of infectious bursal disease virus (IBDV) from bursal tissue by RTPCR and its comparative efficacy with conventional precipitation assays. Indian Journal of Poultry Science, 41 (1). pp. 82-84.

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Abstract

The Infectious bursal disease virus (IBDV) causes considerable morbidity and mortality mainly by immunosuppression in chicken. There are reports of emergence of very virulent lBDV (vvlBDV) strains in different parts of the world including India during the last couple of decades. The present study was aimed at screening IBD suspected bursal samples by reverse transcription-polymerase chain reaction (RT-PCR) and conventional precipitation assays viz. agar gel immunodi ffusion (AGID), and count er immunoelect rophoresis (CIE) to compare their relative sensitivity and specificity. RNA was extracted by Tri Reagent method and cDNA was prepared with OmniscriptTM Reverse Transcriptase Kit. VP) gene specific primers (P1, P 2) were used for generating 643 bp amplification. Out of 37 samples, sensitivity of AGlD and CIE compared to RT-PCR was 74.19 and 87.10 per cent and overall agreement was 78.38 and 89.19 per cent respectively.

EPrint Type:Article
Additional Information:The Infectious bursal disease virus (IBDV) causes considerable morbidity and mortality mainly by immunosuppression in chicken. There are reports of emergence of very virulent lBDV (vvlBDV) strains in different parts of the world including India during the last couple of decades. The present study was aimed at screening IBD suspected bursal samples by reverse transcription-polymerase chain reaction (RT-PCR) and conventional precipitation assays viz. agar gel immunodi ffusion (AGID), and count er immunoelect rophoresis (CIE) to compare their relative sensitivity and specificity. RNA was extracted by Tri Reagent method and cDNA was prepared with OrnniscriptTM Reverse Transcriptase Kit. VP) gene specific primers (P1, P 2) were used for generating 643 bp amplification. Out of 37 samples, sensitivity of AGlD and CIE compared to RT-PCR was 74.19 and 87.10 per cent and overall agreement was 78.38 and 89.19 per cent respectively.
Uncontrolled Keywords:Infectious bursal disease Virus, AGID, CIE, RT-PCR, Poultry
Subjects:Diagnosis > Diagnostic Techniques and Procedures
Animal Diseases > Bird Diseases > Poultry Diseases
Animals > Chordata > Vertebrates > Birds > Poultry
Viruses > RNA Viruses
Biological Sciences > Biotechnology
Diagnosis > Laboratory Techniques and Procedures
Virus Diseases > RNA Virus Infections > Birnaviridae Infections
ID Code:2192
Deposited By:Dr Prakash Koringa
Deposited On:29 June 2007

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