RAPD analysis by PCR using arbitrary primers in different animal species

Joshi, C.G.; Rank, D.N.; Brahmkshtri, B.P.; Patel, A.V.; Vataliya, P.H.; Muraleedharan, P.; Khoda, V.K. and Solanki, J.V. (1998) RAPD analysis by PCR using arbitrary primers in different animal species. Indian Veterinary Journal, 75. pp. 1029-1031.

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Abstract

Molecular genetic technologies have changed the pace and dimension of genetic analysis of livestock species. DNA based polymorphisms are being used for marker assisted selection strategies, parentage testing, species identification, breed characterization and population genetic studies, The advent of RAPD (Randomly amplified polymorphic DNA) by Williams et al. (1990), provided new tool for the molecular geneticist. Amount of polymorphism observed with RAPD is comparable to that of Restriction Fragment Length Polymorphism (RFLP) and microsatelite techniques and possibility of converting an RAPD marker of interest into a single locus PCR marker - SCAR (sequence characterized amplified region) have made HAPD a promising technique.

EPrint Type:Article
Uncontrolled Keywords:RAPD Analysis, Animals, PCR, Arbitrary Primers, Molecular Genetic Techniques, Randomly amplified polymorphic DNA, Buffaloes, Cattle, Dog, Sheep
Subjects:Animals > Chordata > Vertebrates > Mammals > Artiodactyla > Ruminants > Cattle
Animals > Chordata > Vertebrates > Mammals
Investigative Techniques > Genetic Techniques
Animals > Chordata > Vertebrates > Mammals > Artiodactyla > Ruminants > Sheep
Animals > Animal Population Groups
Nucleic Acids, Nucleotides, and Nucleosides > Nucleic Acids > DNA
Genetic Phenomena > Variation (Genetics)
Animals > Chordata > Vertebrates > Mammals > Artiodactyla > Ruminants > Buffaloes
-Journal Repositories > Indian Veterinary Journal
Biological Sciences > Biotechnology
ID Code:2196
Deposited By:Dr Prakash Koringa
Deposited On:28 June 2007

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