Comparison of DNA extraction protocols on frozen stored blood samples
Joshi, C.G.; Rank, D.N.; Brahmkshtri, B.P.; Patel, A.V.; Vataliya, P.H. and Solanki, J.V. (1998) Comparison of DNA extraction protocols on frozen stored blood samples. Indian Veterinary Journal, 75. pp. 1038-1039.
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Two major obstacles encountered when extracting DNA from a large number of samples are the cumbersome methods of deproteinising cell digest with the hazardous-toxic organic solvent (phenol and chloroform) and incomplete recovery of aqueous phase leading to loss of DNA. Phenol-Chloroform extraction is compared with high salt deproteinisation procedure.
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