Comparison of DNA extraction protocols on frozen stored blood samples

Joshi, C.G.; Rank, D.N.; Brahmkshtri, B.P.; Patel, A.V.; Vataliya, P.H. and Solanki, J.V. (1998) Comparison of DNA extraction protocols on frozen stored blood samples. Indian Veterinary Journal, 75. pp. 1038-1039.

Full text available as:

PDF - Requires Adobe Acrobat Reader or other PDF viewer.
51 Kb

Abstract

Two major obstacles encountered when extracting DNA from a large number of samples are the cumbersome methods of deproteinising cell digest with the hazardous-toxic organic solvent (phenol and chloroform) and incomplete recovery of aqueous phase leading to loss of DNA. Phenol-Chloroform extraction is compared with high salt deproteinisation procedure.

EPrint Type:Article
Uncontrolled Keywords:DNA Extraction Protocols, Frozen Blood, Organic Solvent, Phenol, Chloroform
Subjects:Organic Chemicals > Phenols > Phenol
Nucleic Acids, Nucleotides, and Nucleosides > Nucleic Acids > DNA
Hemic and Immune Systems > Blood
Organic Chemicals > Hydrocarbons > Hydrocarbons, Halogenated
-Journal Repositories > Indian Veterinary Journal
Biological Sciences > Biotechnology
ID Code:2199
Deposited By:Dr Prakash Koringa
Deposited On:02 July 2007

Archive Staff Only: edit this record