Development of somatic cell hybrid using buffalo leukocytes and mouse cell line
Rank, D.N.; Joshi, C.G.; Thiagrajan, V.; Kanakraj, P. and Krishnan, A.R. (2004) Development of somatic cell hybrid using buffalo leukocytes and mouse cell line. Indian Veterinary Journal, 81. pp. 156-159.
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Somatic cell hybridization is considered as key discovery in the field of mammalian genetics that opened the avenues for gene mapping in any species. A considerable number of syntenic relationship and gene assignments have been established especially in human and cattle using somatic cell hybridization (O'Brien, 1993). The technique for developing somatic cell hybrid in several bovine species especially cattle and sheep is described in the literature. However, very few reports are available on production of somatic cell hybrids involving buffalo cells. Present study describes variables affecting efficiency of somatic cell hybridization between buffalo leucocytes and RAG, mouse cell line.
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