Comparative evaluation of phenobarbital-induced CYP3A and CYP2H1 gene expression by quantitative RT-PCR in Bantam, Bantamized white leghorn and white leghorn chicks

Goriya, H.V.; Kalia, A.; Bhavsar, S.K.; Joshi, C.G.; Rank, D.N. and Thaker, A.M. (2005) Comparative evaluation of phenobarbital-induced CYP3A and CYP2H1 gene expression by quantitative RT-PCR in Bantam, Bantamized white leghorn and white leghorn chicks. Journal of Veterinary Science, 6 (4). pp. 279-285.

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Abstract

The present work was to study induction of cytochrome P450 (CYP)3A and CYP2H1 gene by reverse transcriptase polymerase chain reaction (RT-PCR) and quantitative RTPCR in Bantam, Bantamized White Leghorn and White Leghorn chicks. Out of 18 chicks total 3 from each group (Bantam, Bantamized White Leghorn and White Leghorn) were treated intraperitoneal with phenobarbital at the dose rate of 12 mg/100 g (body weight) while the control group was treated with the saline. Total RNA was extracted from the liver samples using Tri Reagent based method. First strand cDNA was synthesized using one step RT-PCR kit. The PCR was performed and the product was subjected to agarose gel electrophoresis. Quantitative RT-PCR was conducted to quantify gene expression level of CYP3A and CYP2H1 genes. Relative expression ratio of CYP3A and CYP2H1 genes was calculated using relative expression software tool (REST). It was found that CYP3A is up regulated by factor of 1.34, 14.51 and 1.00 in Bantam, Bantamized White Leghorn and White Leghorn chicks, respectively. In Bantam and Bantamized White Leghorn chicks CYP2H1 gene was up regulated by factor 1.50 and 80.87, respectively but down regulated by a factor of 1.97 in White Leghorn chicks. The PCR efficiency ranged from 1.30 to 1.70, 0.86 to 1.70 and 0.91 to 1.58 for CYP3A, CYP2H1 and β-actin, respectively in Bantam, Bantamized White Leghorn and White Leghorn chicks.

EPrint Type:Article
Uncontrolled Keywords:Cytochrome P450, CYP3A, CYP2H1, Phenobarbital, RT-PCR, White Leghorn Chicks, Bantamized
Subjects:Genetic Processes > Gene Expression
Heterocyclic Compounds > Heterocyclic Compounds, 1-Ring > Pyrimidines
Investigative Techniques > Genetic Techniques
Chemical and Pharmacologic Phenomena > Dose-Response Relationship, Drug
Animals > Chordata > Vertebrates > Birds > Poultry
Digestive System > Liver
-Journal Repositories > Indian Veterinary Journal
ID Code:2208
Deposited By:Dr Prakash Koringa
Deposited On:04 July 2007

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