Prolactin genotyping of Indian buffalo breeds using PCR-RFLP
Ladani, D.D.; Pipalia, D.L.; Brahmkshtri, B.P.; Rank, D.N.; Joshi, C.G.; Vataliya, P.H. and Solanki, J.V. (2003) Prolactin genotyping of Indian buffalo breeds using PCR-RFLP. Buffalo Journal, 2. pp. 203-208.
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Prolactin plays an important regulatory function in milk production and reproduction. Prolactin gene was explored in three buffalo breeds by PCR-RFLP. Genomic DNA was isolated from blood samples of 21 Jaffarabadi, 44 Mehsani and 29 Surti buffaloes (Bubalus bubalis) and also from semen samples of 2 Jaffarabadi bulls. A 156 bp PRL gene exon III segment was amplified by PCR using bovine specific primers. RFLPs in this segment was studied using Rsa I restriction enzyme. Genotype AA was not detected in any of the breeds studied. The frequencies of AB and BB genotypes in Jaffarabadi, Mehsani and Surti buffaloes were 0.87 and 0.13; 1.0 and 0.0; 0.965 and 0.035, respectively. Frequencies of allele A and B were 0.435 and 0.565 in Jaffarabadi; 0.5 each in Mehsani; and 0.482 and 0.518 in Surti respectively.
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