Cytogenetic profile of kachchhi camel (Camelus dromedarius)

Patel, M.K.; Patel, K.S.; Brahmkshtri, B.P.; Vataliya, P.H. and Solanki, J.V. (2000) Cytogenetic profile of kachchhi camel (Camelus dromedarius). Journal of Camel Practice and Research, 7 (2). pp. 201-202.

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Abstract

Cytogenetic study of Kachchhi camel was carried out using short term whole blood culture technique. RPMI-1640 containing 15% foetal bovine serum, pokeweed mitogen (2.5 microgram/ml) and antibiotic was used for the culture. The cell growth was arrested in metaphase using colchicine (0.5 microgram/ml) after 71 hrs. The metaphase spread were obtained after hyypotonic treatment using 0.56 per cent KCL for 10 minutes followed by 3:1 methanol : acetic acid. Giemsa stain karyotpe of male and female kachchhi camel revealed diploid chromosome complement consisting of 31 pairs of acrocentric chromosomes and 5 pairs of submetacentric chromosomes. X chromosome was one of the largest metacentric and chromosome was small metacentric.

EPrint Type:Article
Uncontrolled Keywords:Karyotyping, Camel, Blood Culture Techniques, Bovine Serum, Cytogenetic Study, X-Chromosomes
Subjects:Investigative Techniques > Genetic Techniques
Investigative Techniques > Clinical Laboratory Techniques
Genetic Structures > Chromosomes
Investigative Techniques > Clinical Laboratory Techniques > Culture Techniques
Investigative Techniques > Animal Experimentation
Animals > Chordata > Vertebrates > Mammals > Artiodactyla > Ruminants > Camels
ID Code:2211
Deposited By:Dr Prakash Koringa
Deposited On:06 July 2007

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