Detection of BoLA-DRB3 gene polymorphism in Gir and Kankrej cattle using PCR-RFLP

Acharya, C.P.; Pipalia, D.L.; Rank, D.N.; Joshi, C.G.; Solanki, J.V. and Shah, R.R. (2002) Detection of BoLA-DRB3 gene polymorphism in Gir and Kankrej cattle using PCR-RFLP. Indian Journal of Animal Sciences, 72 (8). pp. 680-683.

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Abstract

BoLA-DRB3 gene polymorphism was investigated in two cattle breeds, Gir and Kankrej by PCR-RFLP. Genomic DNA was isolated from blood samples collected from 57 Gir and 51 Kankrej animals. A 284 bp BoLA-DRB3 exon 2 segment was amplified by heminesting PCR, using the locus specific primes and the PCR products were subjected to Hae III and Rsa I digestion. Digestion with Hae III resulted in three RFLP patterns a, b and d in 5 different combinations (aa, ab, ad, bb and bd) in Gir and 3 RFLP patterns a, b and e in four different combinations (aa, ab, ae, and be) in Kankrej. Pattern a and b were more frequent in both breeds. Rsa I restriction were very complex. The fregments interpretation revelaed 13 different restriction patterns with 25 sombinations in Gir and 10 different combinations in Kankrej. Thus, BoLA-DRB3 was highly polymorphic.

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