Estimation of genetic diversity among Gir, Kankrej and Deoni cattle breeds using microsatellite markers
Kale, Deepak (2004) Estimation of genetic diversity among Gir, Kankrej and Deoni cattle breeds using microsatellite markers. Masters thesis, College of Veterinary Science and Animal Husbandry, Anand Agricultural University, Anand, Gujarat, INDIA.
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Microsatellites or short tandem repeats (STRs), DNA markers relatively abundant in the genome, have a high degree of polymorphism and therefore great potential for characterizing population. The present study describes genetic variability within and between three indigenous cattle breeds viz; Gir Kankrej and Deoni investigated using seven microsatellite markers (ETH- 225, CSRM-60, HEL-5, INRA-005, INRA-035, ILSTS-002 and ILSTS-006). Twenty-two to fifty-two DNA samples per breed isolated from non-related animals were utilized for PCR amplification of microsatellite loci. PCR products were resolved on 7% denaturing polyacrylamide gels and visualized by silver staining. The microsatellite alleles were sized using 10 bp DNA ladder. Allelic frequency, heterozygosity and polymorphic information content (PIC) were estimated. Allelic frequencies were tested for Hardy-Weinberg equilibrium. The results showed that genetic equilibrium was not always maintained. The observed number of alleles ranged from five (in HEL-5) to eight (in CSRM-60) with total 46 alleles across three breeds. The overall heterozygosity and PIC values were 0.730 and 0.749. Genetic distance was least (0.2034) between Gir and Kankrej and highest between Deoni and Kankrej (0.4442). A dendrogram following UPGMA clustering was generated from Nei’s genetic distance matrix using Popgene programme (Version 1.31).The clusters obtained on phylogenetic tree agreed with the geographic origin of the breed.
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