Estimation of genetic diversity among Gir, Kankrej and Deoni cattle breeds using microsatellite markers

Kale, Deepak (2004) Estimation of genetic diversity among Gir, Kankrej and Deoni cattle breeds using microsatellite markers. Masters thesis, College of Veterinary Science and Animal Husbandry, Anand Agricultural University, Anand, Gujarat, INDIA.

Full text available as:

PDF - Requires Adobe Acrobat Reader or other PDF viewer.
735 Kb

Abstract

Microsatellites or short tandem repeats (STRs), DNA markers relatively abundant in the genome, have a high degree of polymorphism and therefore great potential for characterizing population. The present study describes genetic variability within and between three indigenous cattle breeds viz; Gir Kankrej and Deoni investigated using seven microsatellite markers (ETH- 225, CSRM-60, HEL-5, INRA-005, INRA-035, ILSTS-002 and ILSTS-006). Twenty-two to fifty-two DNA samples per breed isolated from non-related animals were utilized for PCR amplification of microsatellite loci. PCR products were resolved on 7% denaturing polyacrylamide gels and visualized by silver staining. The microsatellite alleles were sized using 10 bp DNA ladder. Allelic frequency, heterozygosity and polymorphic information content (PIC) were estimated. Allelic frequencies were tested for Hardy-Weinberg equilibrium. The results showed that genetic equilibrium was not always maintained. The observed number of alleles ranged from five (in HEL-5) to eight (in CSRM-60) with total 46 alleles across three breeds. The overall heterozygosity and PIC values were 0.730 and 0.749. Genetic distance was least (0.2034) between Gir and Kankrej and highest between Deoni and Kankrej (0.4442). A dendrogram following UPGMA clustering was generated from Nei’s genetic distance matrix using Popgene programme (Version 1.31).The clusters obtained on phylogenetic tree agreed with the geographic origin of the breed.

EPrint Type:Thesis (Masters)
Uncontrolled Keywords:Microsatellites, Genetic Variability, Polymorphism, Cattle Breeds, PCR, DNA, Heterozygosity
Subjects:Genetic Structures > Genome Components
-Institutional Repositories > College of Veterinary Science and Animal Husbandry (Anand Agricultural University)
Animals > Chordata > Vertebrates > Mammals > Artiodactyla > Ruminants > Cattle
Genetic Structures > Base Sequence
Investigative Techniques > Genetic Techniques
Immunologic and Biological Factors > Biological Factors > Biological Markers
Biochemical Phenomena, Metabolism, and Nutrition > Biochemical Phenomena
Nucleic Acids, Nucleotides, and Nucleosides > Nucleic Acids > DNA
Genetic Structures > Genome
Genetic Phenomena > Variation (Genetics)
ID Code:2228
Deposited By:Dr Prakash Koringa
Deposited On:19 July 2007

Archive Staff Only: edit this record