Molecular characterization of Surti breed of goat using microsatellite marker

Patel, Mayur (2004) Molecular characterization of Surti breed of goat using microsatellite marker. Masters thesis, College of Veterinary Science and Animal Husbandry, Anand Agricultural University, Anand, Gujarat, INDIA.

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Abstract

It is essential to characterize the germplasm for intragenetic variability, which will help in planning for conservation strategy as well as genetic improvement. Microsatellite markers are widely accepted as a marker of choice for genetic characterization of populations as they are highly polymorphic single locus DNA sequences scaterred throughout the genome and are readily adaptable to Polymerase Chain Reaction (PCR). Present study is an attempt to characterize Surti goat breed at genetic level using fifteen pairs of bovine microsatellite markers. Blood samples were collected randomly from sixty animals of Surti goat from its home track. These samples were assessed for genetic variation at fifteen microsatellite loci using bovine microsatellite primer pairs chosen from the list suggested by International Society for Animal Genetics (ISAG) viz., ETH -152, ETH -225, ILSTS -005, -011, -019, -022, -028, -030, -033, -034, -044, -058, -059, -065, and -087. All the primer pairs successfully amplified goat microsatellite loci. The PCR products were checked on 2% agarose for verification of quantity and size of amplicon. PCR amplification of microsatellite loci on surti goat DNA conformed the size published for other goat breed. The median fragment length of different microsatellite were found to be approximately 203 bp, 153 bp, 182 bp, 276bp, 153 bp, 195 bp, 156 bp, 166 bp, 174 bp, 158 bp, 154 bp, 180 p, 156 bp, 120 bp and 154 bp for the above referred microsatellites respectively. Further the amplicons were run on sequencing polyacrylamide gel electrophoreses (denaturing PAGE) for allele sizing and genotyping. Genetic parameters of genetic variability were estimated from the resultant data. Microsatellite ETH -152, ETH -225, ILSTS -005, -011, -019, -022, -028, -030, -033, -034, -044, -058, -059, -065 and -087 showed 2, 4, 3, 5, 6, 4, 11, 7, 9, 5, 6, 8, 4, 2 and 9 alleles as well as 2, 4, 3, 9, 17, 7, 23, 18, 14, 6, 9, 21, 7, 3 and 26 genotypic combinations, respectively. The heterozygosity values for these microsatellites were 0.200, 0.271, 0.280, 0.543, 0.690, 0.534, 0.692, 0.720, 0.660, 0.288, 0.290, 0.690, 0.690, 0.283 and 0.640 respectively. Polymorphic information content (PIC) was estimated to be 0.164, 0.233, 0.236, 0.540, 0.754, 0.538, 0.737, 0.783, 0.624, 0.270, 0.327, 0.686, 0.493, 0.267 and 0.854 for these microsatellites respectively. All the microsatellites investigated were found to be highly polymorphic with 2 (ETH -152 & ILSTS -065) to 11(ILSTS -028) alleles, 0.200 (ETH -152) to 0.720 (ILSTS -030) heterozygosity and 0.164 (ETH -152) to 0.854 (ILSTS -087) PIC in the Surti goat. These estimates indicated high genetic variability in Surti goat. This is the first reported molecular characterization study on Surti goat.

EPrint Type:Thesis (Masters)
Uncontrolled Keywords:Microsatellite markers, Goat, Surti Breed, PCR, Molecular Characterization
Subjects:Genetic Structures > Genome Components
-Institutional Repositories > College of Veterinary Science and Animal Husbandry (Anand Agricultural University)
Animals > Chordata > Vertebrates > Mammals > Artiodactyla > Ruminants > Goats
Genetic Structures > Base Sequence
Investigative Techniques > Genetic Techniques
Nucleic Acids, Nucleotides, and Nucleosides > Nucleic Acids > DNA
Biological Sciences > Biotechnology
ID Code:2234
Deposited By:Dr Prakash Koringa
Deposited On:23 July 2007

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