Surveillance of haemorrhagic septicemia in Gujarat state with isolation, biochemical characterization and PCR based detection of Pasteurella multocida from the field outbreaks

Nadodha, J.V. (2004) Surveillance of haemorrhagic septicemia in Gujarat state with isolation, biochemical characterization and PCR based detection of Pasteurella multocida from the field outbreaks. Masters thesis, College of Veterinary Science and Animal Husbandry, Anand Agricultural University, Anand, Gujarat, INDIA.

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Abstract

The present research work was undertaken with a view to study the surveillance of Haemorrhagic Septicemia in relation to agro-climatic zones of Gujarat State during the period of January 1998 to December 2003 with isolation and identification of Pasteurella multocida from the field outbreaks in cattle and buffaloes. The isolates were studied for their in vitro antibiotic sensitivity. The PM-PCR was also tried for detection of P.multocida. The data of the study period were collected from the respective sources. The data were compiled and distributed year, month and zone wise. Incidences of HS were correlated to meteorological parameters of different zones. A total of 226 clinical samples (109 each of blood and nasal swabs and eight morbid material) were collected from suspected cases of HS in cattle and buffaloes and processed for demonstration of bipolar organisms and isolation of P.multocida. The isolates were identified by cultural, morphological and biochemical characters. The isolates were subjected to in vitro antibiotic sensitivity. The colony PCR was carried out for detection of P.multocida. The highest number of HS outbreaks were recorded during the year 1998 and lowest were found in 2000. The outbreaks were observed throughout the year but more number of outbreaks were found during the rainy season i.e. months of August and September. Among the agro-climatic zones maximum number of outbreaks were recorded in Zone-IV, whereas minimum in Zone-VII. Positive correlation was observed between outbreaks of HS and rainfall, relative humidity and minimum temperature. While no correlation was observed with outbreaks of HS and maximum temperature. A total of four isolates of P.multocida were recovered only from buffaloes blood which showed the presence of bipolar organisms in smear. All the isolates produced non-haemolytic, round, grayish, smooth and mucoid colonies on blood agar and failed to grow on MacConkey agar. The isolates were found non-motile, Gram negative, coccobacillary rods. All the isolates produced oxidase, catalse, indole and reduced nitrate but did not utilised citrate. They fermented glucose, sucrose, mannitol and mannose but nor fermented maltose, arabinose, lactose, dulcitol, salicin, inositol and trehalose. All the isolates were found sensitive to gentamicin, chloramphenicol and cephalexin, while two isolates were sensitive to tetracycline, ampicillin andnitrofurantoin. All the isolates were found to be resistant against penicillin-G and streptomycin. All the P.multocida isolates along with P52 vaccine strain amplified product of approximately 465 bp size while E.coli failed to amplify.

EPrint Type:Thesis (Masters)
Additional Information:Haemorrhagic Septicemia (HS) - Polymerase Chain Reaction (PCR)- Field Outbreak - Biochemical - Molecular - Charcterization
Uncontrolled Keywords:Haemorrhagic Septicemia, Pasteurella multocida, Gujarat, Buffaloes, Isolation, PCR
Subjects:-Institutional Repositories > College of Veterinary Science and Animal Husbandry (Anand Agricultural University)
Animals > Chordata > Vertebrates > Mammals > Artiodactyla > Ruminants > Cattle
Equipment and Supplies > Culture Media
Investigative Techniques > Genetic Techniques
Investigative Techniques > Clinical Laboratory Techniques > Culture Techniques
Animal Diseases > Cattle Diseases > Hemorrhagic Syndrome, Bovine
Animals > Chordata > Vertebrates > Mammals > Artiodactyla > Ruminants > Buffaloes
Biological Sciences > Biotechnology
Animal Diseases > Cattle Diseases > Pasteurellosis, Pneumonic
ID Code:2235
Deposited By:Dr Prakash Koringa
Deposited On:23 July 2007

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