Molecular characterization of "Kathiawari" breed of horse using microsatellite markers

Koringa, Prakash (2005) Molecular characterization of "Kathiawari" breed of horse using microsatellite markers. Masters thesis, College of Veterinary Science and Animal Husbandry, Anand Agricultural University, Anand, Gujarat, INDIA.

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Abstract

It is essential to characterize the germplasm for introgenetic variability, which will help in planning for conservation strategy as well as genetic improvement. Microsatellite markers are widely accepted as a marker of choice as they are highly polymorphic single locus DNA sequenced throughout the genome and are readily adaptable to Polymerase Chain Reaction (PCR). Present study is an attempt to characterize Kathiawari horse breed using fifteen sets of equine microsatellite markers. DNA samples were taken from sixty horses from its native tract. These samples were assessed for genetic variation using fifteen equine specific microsatellite markers viz. loci NVHEQ 05, NVHEQ 21, NVHEQ 54, NVHEQ 79, LEX 20, HTG 07, NVHEQ 29, ASB 02, NVHEQ 18, HMS 03, HMS 07, NVHEQ 11, VHL 20, HTG 14 and HTG 15. All samples did not respond to PCR amplification. Forty seven to fifty eight samples yielded amplification. The PCR products were checked on 2% agarose for amplification. Further the amplicons were run on sequencing PAGE (Denaturing PAGE) with 10 bp ladder for allele sizing and allele typing. The sizes (length) of different microsatellites were found to be approximately 153bp, 174bp, 173bp, 176bp, 216bp, 142bp, 122bp, 200bp, 137bp, 166bp, 178bp, 126bp, 97bp, 142bp, and 144bp for microsatellites NVHEQ 05, NVHEQ 21, NVHEQ 54, NVHEQ 79, LEX 20, HTG 07, NVHEQ 29, ASB 02, NVHEQ 18, HMS 03, HMS 07, NVHEQ 11, VHL 20, HTG 14 and HTG 15, respectively. A wide range of genetic analyses were then performed on the resultant data. A high level of genetic variation was observed. Microsatellites NVHEQ 05, NVHEQ 21, NVHEQ 54, NVHEQ 79, LEX 20, HTG 07, NVHEQ 29, ASB 02, NVHEQ 18, HMS 03, HMS 07, NVHEQ 11, VHL 20, HTG 14 and HTG 15 showed 4, 4, 2, 5, 6, 4, 5, 5, 8, 8, 6, 6, 8, 4 and 3 alleles as well as 7, 7, 2, 7, 13, 6, 12, 9, 11, 20, 14, 10, 20, 9 and 5 genotypic combinations, respectively. The heterozygosity values in these microsatellites were 0.5306, 0.5745, 0.1400, 0.6400, 0.8600, 0.5472, 0.8200, 0.8200, 0.6792, 0.7959, 0.7241, 0.8478, 0.8235, 0.7400 and 0.5185 for microsatellites NVHEQ 05, NVHEQ 21, NVHEQ 54, NVHEQ 79, LEX 20, HTG 07, NVHEQ 29, ASB 02, NVHEQ 18, HMS 03, HMS 07, NVHEQ 11, VHL 20, HTG 14 and HTG 15 respectively. Microsatellite LEX 20 was highly polymorphic and NVHEQ 54 was least polymorphic among all fifteen microsatellites. Polymorphic information content (PIC) was calculated from number of alleles and heterozygosity. It was 0.441, 0.485, 0.122, 0.483, 0.737, 0.434, 0.732, 0.664, 0.711, 0.752, 0.707, 0.699, 0.820, 0.682 and 0.377 for microsatellite NVHEQ 05, NVHEQ 21, NVHEQ 54, NVHEQ 79, LEX 20, HTG 07, NVHEQ 29, ASB 02, NVHEQ 18, HMS 03, HMS 07, NVHEQ 11, VHL 20, HTG 14 and HTG 15 respectively. The microsatellites were found to be highly polymorphic with 2 to 8 alleles, 0.1400 to 0.8600 heterozygosity and 0.122 to 0.820 PIC in the Kathiawari horse. This is the first reported molecular characterization study on Kathiawari horse.

EPrint Type:Thesis (Masters)
Uncontrolled Keywords:Microsatellite is the most videly used molecular marker for characterization of livestock breeds
Subjects:-Institutional Repositories > College of Veterinary Science and Animal Husbandry (Anand Agricultural University)
Chemical Actions and Uses > Pharmacologic Actions > Molecular Mechanisms of Action
Investigative Techniques > Molecular Probe Techniques
Investigative Techniques > Genetic Techniques
Biochemical Phenomena, Metabolism, and Nutrition > Biochemical Phenomena
Genetic Phenomena > Variation (Genetics)
Biological Sciences > Biotechnology
ID Code:2257
Deposited By:Dr Prakash Koringa
Deposited On:25 July 2007

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