Microsatellite markers for genetic characterization of Kachchhi camel

Mehta, S.C.; Goyal, A. and Sahani, M.S. (2007) Microsatellite markers for genetic characterization of Kachchhi camel. Indian Journal of Biotechnology, 16 (7). pp. 336-339. ISSN 0972-5849

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Abstract

Sixteen microsatellite loci were investigated for studying the genetic polymorphism in Kachchhi breed of camel. Polymerase chain reactions were carried out for 50 unrelated camels of Kachchhi breed. The amplification products were resolved in 6% (denaturing) urea PAGE and stained with silver nitrate. Out of the 16 microsatellite loci, 13 were found polymorphic. The number of alleles ranged from 2 to 6. The expected heterozygosity ranged from 0.332 to 0.796. The polymorphic information content ranged from 0.277 to 0.765. The results indicated existence of enough genetic variation among dromedary individuals and the potential use of microsatellite markers for further genetic investigations including genetic diversity analysis, individual identification, parentage testing and production enhancement.

EPrint Type:Article
Additional Information:Kachchhi breed of camel is known for the production of milk and draught potential
Uncontrolled Keywords:Breeds, Camel, Genetic Characterization, Kachchhi, Microsatellite, Genetic Polymorphism, Polymerase Chain Reaction
Subjects:Genetic Structures > Genome Components
Investigative Techniques > Genetic Techniques
Genetic Structures > Base Sequence
Animals > Animal Population Groups
Genetic Phenomena > Variation (Genetics)
Animals > Chordata > Vertebrates > Mammals > Artiodactyla > Ruminants > Camels
ID Code:2395
Deposited By:Dr S.C. Mehta
Deposited On:12 October 2007

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