Microsatellite markers for genetic characterization of Kachchhi camel
Mehta, S.C.; Goyal, A. and Sahani, M.S. (2007) Microsatellite markers for genetic characterization of Kachchhi camel. Indian Journal of Biotechnology, 16 (7). pp. 336-339. ISSN 0972-5849
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Sixteen microsatellite loci were investigated for studying the genetic polymorphism in Kachchhi breed of camel. Polymerase chain reactions were carried out for 50 unrelated camels of Kachchhi breed. The amplification products were resolved in 6% (denaturing) urea PAGE and stained with silver nitrate. Out of the 16 microsatellite loci, 13 were found polymorphic. The number of alleles ranged from 2 to 6. The expected heterozygosity ranged from 0.332 to 0.796. The polymorphic information content ranged from 0.277 to 0.765. The results indicated existence of enough genetic variation among dromedary individuals and the potential use of microsatellite markers for further genetic investigations including genetic diversity analysis, individual identification, parentage testing and production enhancement.
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