Genetic characterization of Jaisalmeri camel using microsatellite markers

Gautam, L.; Mehta, S.C.; Gahlot, R.S. and Gautam, K. (2004) Genetic characterization of Jaisalmeri camel using microsatellite markers. Indian Journal of Biotechnology, 4. pp. 457-459. ISSN 0972-5849

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Six New World Camelidae microsatellite primer pairs were used to investigate the genetic polymorphism in Jaisalmeri camel. Polymerase chain reactions were carried out for 30 unrelated camels of Jaisalmeri breed. The amplification products were resolved in 6% (denaturing) urea PAGE and stained with silver nitrate. All six microsatellite primer pairs were found polymorphic in Jaisalmeri camel. The number of alleles ranged from 2 to 5. The expected heterozygosity ranged from 0.32 to 0.651 and the polymorphic information content ranged from 0.268 to 0.588. The results indicated the utility of these microsatellite loci for studying genetic polymorphism in dromedary breeds.

EPrint Type:Article
Additional Information:Jaisalmeri breed of camel is well known for the race potential.
Uncontrolled Keywords:Microsatellite Markers, Jaisalmeri Camel, Breeds, Hetrozygotes, Genetic Pokymorphism, Polymerase Chain Reaction
Subjects:Investigative Techniques > Genetic Techniques
Genetic Structures > Base Sequence
Genetic Phenomena > Variation (Genetics)
Genetic Processes > Breeding
Animals > Chordata > Vertebrates > Mammals > Artiodactyla > Ruminants > Camels
ID Code:2397
Deposited By:Dr S.C. Mehta
Deposited On:16 October 2007

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