A rapid radiometric biphasic vial system for detection and testing drug susceptibility of Mycobacterium tuberculosis using 14c-labelled acetate
Deodhar, M.N.; Shah, D.H.; Ganatra, R.D.; Kale, R.N.; Virdi, S.S. and Deshmukh, M.D. (1983) A rapid radiometric biphasic vial system for detection and testing drug susceptibility of Mycobacterium tuberculosis using 14c-labelled acetate. Indian Journal of Tuberculosis, Supplement, 30 (3). pp. 45-48.
Full text available as:
A simple and rapid radiorespirometric method, for early detection and testing of drug susceptibility of tubercle bacilli from sputum samples has been developed. A biphasic liquid scintillation vial system is used for detection of 14 CO2. produced by metabolism of acetate 1-2 l4 C1 on glycerol free Lowenstein-Jensen Media (LJM). Eighty four smear positive sputum samples were tested by radiometric method (RM) and visual growth method (VM). Seventy two samples were detected positive by RM, while 73 were scored positive by VM. The average detection time by RM was 10.7 days, compared lo 21 days by VM. The drug susceptibility test was carried out on 7 of the radiometric positive samples, using streptomycin, isoniazid, rifampicin and ethambutol, each tested at three different concentrations. Radiometric results were available at 72-96 hours as compared to 3 weeks by visual method.
Archive Staff Only: edit this record