A rapid radiometric biphasic vial system for detection and testing drug susceptibility of Mycobacterium tuberculosis using 14c-labelled acetate

Deodhar, M.N.; Shah, D.H.; Ganatra, R.D.; Kale, R.N.; Virdi, S.S. and Deshmukh, M.D. (1983) A rapid radiometric biphasic vial system for detection and testing drug susceptibility of Mycobacterium tuberculosis using 14c-labelled acetate. Indian Journal of Tuberculosis, Supplement, 30 (3). pp. 45-48.

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Abstract

A simple and rapid radiorespirometric method, for early detection and testing of drug susceptibility of tubercle bacilli from sputum samples has been developed. A biphasic liquid scintillation vial system is used for detection of 14 CO2. produced by metabolism of acetate 1-2 l4 C1 on glycerol free Lowenstein-Jensen Media (LJM). Eighty four smear positive sputum samples were tested by radiometric method (RM) and visual growth method (VM). Seventy two samples were detected positive by RM, while 73 were scored positive by VM. The average detection time by RM was 10.7 days, compared lo 21 days by VM. The drug susceptibility test was carried out on 7 of the radiometric positive samples, using streptomycin, isoniazid, rifampicin and ethambutol, each tested at three different concentrations. Radiometric results were available at 72-96 hours as compared to 3 weeks by visual method.

EPrint Type:	Article
Uncontrolled Keywords:	Radiorespirometric Method, Tubercule bacilli, Drug Susceptibility, Sputum, Lowenstein-Jensen Media, Streptomycin, Isoniazid, Rifampicin, Ethambutol, Radiometry
Subjects:	Natural Sciences > Physics > Radiation Organic Chemicals > Hydrazines > Isoniazid Inorganic Chemicals > Isotopes > Carbon Isotopes Heterocyclic Compounds > Heterocyclic Compounds with 4 or More Rings > Rifamycins Immunologic Diseases > Hypersensitivity > Drug Hypersensitivity Organic Chemicals > Carboxylic Acids > Acids, Acyclic Fluids and Secretions > Bodily Secretions > Sputum Diagnosis > Diagnostic Techniques and Procedures Equipment and Supplies > Culture Media Bacteria > Gram-Positive Bacteria > Actinobacteria > Actinomycetales > Mycobacteriaceae > Mycobacterium > Mycobacterium tuberculosis Organic Chemicals > Amines > Polyamines Investigative Techniques > Epidemiologic Methods Carbohydrates and Hypoglycemic Agents > Carbohydrates > Glycosides > Aminoglycosides > Streptomycin Environment and Public Health > Public Health > Epidemiologic Methods Investigative Techniques > Radiometry -Journal Repositories > Indian Journal of Tuberculosis
ID Code:	2678
Deposited By:	Mrs Naina Pandita
Deposited On:	05 March 2008

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