Evaluation of radiommunoassay for T.B. antigen in clinical situations

Samuel, A.M.; Kadival, G.V.; Ganatra, R.D.; Irani, S.; Pandya, S.; Virdi, B.S. and Deshmukh, M.D. (1983) Evaluation of radiommunoassay for T.B. antigen in clinical situations. Indian Journal of Tuberculosis, Supplement, 30 (3). 49.

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Abstract

A definite diagnosis of tuberculosis depends on the demonstration of the presence of organism either by microscopy or by culture. Isolation of organisms by culture is usually time consuming and often unrewarding. Serological kits for antibody in serum for diagnostic purposes has not been useful as antibody levels persist long after the disease has subsided. The present study describes a RIA for the detection of tubercular antigen using 37H Rv sonicates, Anti Bca antibody and a double antibody separation procedure. The assay sensitivity was 1 x 1C 3 / ml or an equivalent of 1 nb/ml of sonicate antigen. There was very little (5%) cross reactivity against other Myco-bacterial species except Mycobact. bovis where cross reactivity was neatly 100%.

EPrint Type:Article
Uncontrolled Keywords:Tuberculosis, Diagnosis, Tubercular Antigen, Mycobacterium bovis, Serological kits, Tubercular meningitis, Ascitic Fluid, Pleural Fluid, Radioimmunoassay
Subjects:Bacterial Infections and Mycoses > Bacterial Infections > Gram-Positive Bacterial Infections > Actinomycetales Infections > Mycobacterium Infections > Tuberculosis
Nervous System Diseases > Central Nervous System Diseases > Meningitis
Fluids and Secretions > Body Fluids
Respiratory Tract Diseases > Pleural Diseases > Pleural Effusion
Investigative Techniques > Immunologic Techniques
Nervous System Diseases > Central Nervous System Diseases
Fluids and Secretions > Bodily Secretions > Sputum
Diagnosis > Diagnostic Techniques and Procedures
Bacteria > Gram-Positive Bacteria > Actinobacteria > Actinomycetales > Mycobacteriaceae > Mycobacterium > Mycobacterium bovis
Bacteria > Gram-Positive Bacteria > Actinobacteria > Actinomycetales > Mycobacteriaceae > Mycobacterium > Mycobacterium tuberculosis
Immunologic and Biological Factors > Immunologic Factors > Antigens > Antigens, Bacterial
-Journal Repositories > Indian Journal of Tuberculosis
Diagnosis > Laboratory Techniques and Procedures
ID Code:2679
Deposited By:Mrs Naina Pandita
Deposited On:05 March 2008

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