Evaluation of radiommunoassay for T.B. antigen in clinical situations
Samuel, A.M.; Kadival, G.V.; Ganatra, R.D.; Irani, S.; Pandya, S.; Virdi, B.S. and Deshmukh, M.D. (1983) Evaluation of radiommunoassay for T.B. antigen in clinical situations. Indian Journal of Tuberculosis, Supplement, 30 (3). 49.
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A definite diagnosis of tuberculosis depends on the demonstration of the presence of organism either by microscopy or by culture. Isolation of organisms by culture is usually time consuming and often unrewarding. Serological kits for antibody in serum for diagnostic purposes has not been useful as antibody levels persist long after the disease has subsided. The present study describes a RIA for the detection of tubercular antigen using 37H Rv sonicates, Anti Bca antibody and a double antibody separation procedure. The assay sensitivity was 1 x 1C 3 / ml or an equivalent of 1 nb/ml of sonicate antigen. There was very little (5%) cross reactivity against other Myco-bacterial species except Mycobact. bovis where cross reactivity was neatly 100%.
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