Serogroups, atypical biochemical characters, colicinogeny and antibiotic resistance pattern of shiga toxin-producing Escherichia coli isolated from diarrhoeic calves in Gujarat, India

Arya, Gitanjali; Roy, Ashish; Chaudhary, Vandana; Yadav, Mahendra M. and Joshi, C.G. (2008) Serogroups, atypical biochemical characters, colicinogeny and antibiotic resistance pattern of shiga toxin-producing Escherichia coli isolated from diarrhoeic calves in Gujarat, India. Zoonoses Public Health, 55 (2008). pp. 89-98.

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Abstract

This study was designed to investigate the antibiotic resistance, colicinogeny, serotyping and atypical biochemical characteristics of 41 Shiga toxin-producing Escherichia coli (STEC) strains detected using polymerase chain reaction from 90 E. coli strains isolated from 46 diarrhoeic calves. The STEC strains belonged to 14 different serogroups. Seventeen per cent of the STEC strains carried the eaeA gene while 14.28% of the 49 non-STEC strains were eaeA positive. Twenty eight (68.29%) of the 41 STEC strains were rhamnose non-fermentors. All the STEC strains revealed resistance to at least three of the antibiotics tested. 100% resistance was found against kanamycin and cephalexin followed by cephaloridine, enrofloxacin, amikacin, ampicillin, tetracycline, ceftiofur, ciprofloxacin, colistin and co-trimoxazole. Eighteen (44%) of the STEC strains produced colicin and all these colicinogenic strains were resistant to three or more antibiotics. Eleven STEC strains (26.82%) showed urease activity. The results of this study suggest that diarrhoeic calves are an important reservoir of STEC strains that are potentially pathogenic for farm animals and humans. Moreover, rhamnose fermentation, colicinogeny and atypical biochemical behaviour, such as urease activity, may serve as important markers or diagnostic tools for epidemiological surveys to trace the source of infection in disease outbreaks.

EPrint Type:Article
Uncontrolled Keywords:Shiga toxin-producing E. coli; Calf Diarrhoea, India; Colicinogeny; Antibiotic Resistance, Escherichia coli, Kanamycin, Cephalexin, Cephaloridine, Enrofloxacin, Amikacin, Ampicillin, Tetracycline, Ceftiofur, Ciprofloxacin, Colistin, Co-trimoxazole.
Subjects:-Institutional Repositories > College of Veterinary Science and Animal Husbandry (Anand Agricultural University)
Bacteria > Proteobacteria
Animals > Chordata > Vertebrates > Mammals > Artiodactyla > Ruminants > Cattle
Chemical Actions and Uses > Pharmacologic Actions > Therapeutic Uses > Anti-Infective Agents > Anti-Bacterial Agents
Chemical and Pharmacologic Phenomena > Drug Resistance
Enzymes and Coenzymes > Enzymes > Hydrolases
Genetic Structures > Attachment Sites (Microbiology)
Immunologic and Biological Factors > Biological Factors > Toxins
Pathological Conditions, Signs and Symptoms > Signs and Symptoms > Signs and Symptoms, Digestive
Bacteria > Gram-Negative Bacteria
Biological Sciences > Biotechnology
ID Code:2696
Deposited By:Dr Prakash Koringa
Deposited On:10 March 2008

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