Genetic characterization of Indian peste des petits ruminants virus (PPRV) by sequencing and phylogenetic analysis of fusion protein and nucleoprotein gene segments

Kerur, N.; Jhala, M.K. and Joshi, C.G. (2007) Genetic characterization of Indian peste des petits ruminants virus (PPRV) by sequencing and phylogenetic analysis of fusion protein and nucleoprotein gene segments. Research in Veterinary Science, 85 (1). pp. 176-183.

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Abstract

Peste des petits ruminants (PPR) is an important viral disease of sheep and goats, endemic in India. The study was undertaken to characterize the local PPRV by sequencing fusion (F) protein and nucleoprotein (N) gene segments and phylogenetic analysis, so as to focus on genetic variation in the field viruses. Selected regions of PPRV genome were amplified from clinical samples collected from 32 sheep and goats by RT-PCR and the resulting amplicons were sequenced for phylogenetic analysis. The phylogenetic tree based on the 322 bp F gene sequences of PPRV from five different locations clustered them into lineage 4 along with other Asian isolates. While the 425 bp N gene sequences revealed a different pattern of branching, yielding three distinct clusters for Nigerian, Turkey and Indian isolates. Thus, classification of PPRV into lineages based on the N gene sequences appeared to yield better picture of molecular epidemiology for PPRV.

EPrint Type:Article
Uncontrolled Keywords:Peste des Petits Ruminants, Goats, Sheep, PPRV Sequencing, Nucleoprotein, Genome, Phylogenetic Analysis
Subjects:Animal Diseases > Peste-des-Petits-Ruminants
-Institutional Repositories > College of Veterinary Science and Animal Husbandry (Anand Agricultural University)
Genetic Structures > Genes
Animals > Chordata > Vertebrates > Mammals > Artiodactyla > Ruminants > Goats
Amino Acids, Peptides, and Proteins > Proteins > Nucleoproteins
Animals > Chordata > Vertebrates > Mammals > Artiodactyla > Ruminants > Sheep
Nucleic Acids, Nucleotides, and Nucleosides > Nucleic Acids > DNA
Biological Sciences > Biotechnology
ID Code:2920
Deposited By:Dr Prakash Koringa
Deposited On:16 June 2008

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