The effect of storage of tubercle bacilli in transport medium and simplified culture technique
Vasanthakumari, R.; Jagannath, K. and Rajashekaran, Mr. (1987) The effect of storage of tubercle bacilli in transport medium and simplified culture technique. Indian Journal of Tuberculosis, 37 (3). pp. 143-146.
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Two hundred and fifty sputum samples were cultured by a simple method using a solution of trisodium phosphate and the results were compared with those of Petroff’s method. A statistically significant higher culture positivity and a lower contamination rate were obtained in the new method. To test the efficacy of trisodium phosphate solutions a second study was conducted on 175 positive sputa. Sputum samples were collected in this medium and stored upto six days at room temperature and cultured by the simple method. A significant fall in culture positivity after three days of storage and smear positivity after five days was seen as against two days and more than six days, respectively) with plain sputum.
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