The effect of storage of tubercle bacilli in transport medium and simplified culture technique

Vasanthakumari, R.; Jagannath, K. and Rajashekaran, Mr. (1987) The effect of storage of tubercle bacilli in transport medium and simplified culture technique. Indian Journal of Tuberculosis, 37 (3). pp. 143-146.

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Abstract

Two hundred and fifty sputum samples were cultured by a simple method using a solution of trisodium phosphate and the results were compared with those of Petroff’s method. A statistically significant higher culture positivity and a lower contamination rate were obtained in the new method. To test the efficacy of trisodium phosphate solutions a second study was conducted on 175 positive sputa. Sputum samples were collected in this medium and stored upto six days at room temperature and cultured by the simple method. A significant fall in culture positivity after three days of storage and smear positivity after five days was seen as against two days and more than six days, respectively) with plain sputum.

EPrint Type:Article
Uncontrolled Keywords:Tubercule Bacilli, Sputum, Culture, Culture Technique, Transport Medium, Contamination, Petroff's Method, Trisodium Phosphate
Subjects:Inorganic Chemicals > Electrolytes > Ions
Fluids and Secretions > Bodily Secretions > Sputum
Equipment and Supplies > Culture Media
Bacteria > Gram-Positive Bacteria > Actinobacteria > Actinomycetales > Mycobacteriaceae > Mycobacterium > Mycobacterium tuberculosis
Respiratory Tract Diseases > Respiratory Tract Infections > Tuberculosis, Pulmonary
Investigative Techniques > Clinical Laboratory Techniques > Culture Techniques
Inorganic Chemicals > Phosphorus Compounds > Phosphorus Acids
Respiratory Tract Diseases > Lung Diseases > Tuberculosis, Pulmonary
-Journal Repositories > Indian Journal of Tuberculosis
Inorganic Chemicals > Sodium Compounds
ID Code:3104
Deposited By:Mrs Naina Pandita
Deposited On:06 October 2008

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