Enzyme immunoassay based study of IgG and IgM antibody response to antigens of M. tuberculosis (H37 Rv) in patients with pulmonary tuberculosis

Agarwal, Alka and Moudgil, K.D. (1988) Enzyme immunoassay based study of IgG and IgM antibody response to antigens of M. tuberculosis (H37 Rv) in patients with pulmonary tuberculosis. Indian Journal of Tuberculosis, 35 (1). pp. 12-16.

Full text available as:

PDF - Requires Adobe Acrobat Reader or other PDF viewer.
474 Kb

Abstract

Enzyme immunoassays based on three different antigenic preparations of M. tuberculosis (H37 Rv), i.e. intact cells (1C), sonicate supernatant (SS) and purified protein derivative (PPD), were developed. The assays were used for titration of IgG or IgM antibodies in sera of patients with active pulmonary tuberculosis and normal healthy individual. The tuberculosis sera showed predominantly IgG type of response against 1C, SS and PPD, and the mean IgG reactivity against these antigens was 1,45, 13 and 9 fold higher respectively, as compared to normal controls. On the basis of IgG reactivity, SS and PPD could differentiate 80 7 and 94.73 percent of tuberculosis patients respectively, from normal controls. The IgM response of tuberculosis patients was relatively much less as compared to IgG response, and had no discriminatory significance.

EPrint Type:	Article
Uncontrolled Keywords:	Enzyme Immunoassays, Pulmonary Tuberculosis, IgG, IgM, Antigens, Antibodies, Serum, Mycobacterium tuberculosis, Patients, ELISA
Subjects:	Immunologic and Biological Factors > Immunologic Factors > Antibodies Investigative Techniques > Immunologic Techniques Hemic and Immune Systems > Blood Persons > Patients Amino Acids, Peptides, and Proteins > Proteins > Blood Proteins Bacteria > Gram-Positive Bacteria > Actinobacteria > Actinomycetales > Mycobacteriaceae > Mycobacterium > Mycobacterium tuberculosis Immunologic and Biological Factors > Immunologic Factors > Antigens > Antigens, Bacterial Respiratory Tract Diseases > Lung Diseases > Tuberculosis, Pulmonary -Journal Repositories > Indian Journal of Tuberculosis Investigative Techniques > Molecular Probe Techniques Diagnosis > Laboratory Techniques and Procedures
ID Code:	3128
Deposited By:	Mrs Naina Pandita
Deposited On:	03 November 2008

Archive Staff Only: edit this record