Enzyme immunoassay based study of IgG and IgM antibody response to antigens of M. tuberculosis (H37 Rv) in patients with pulmonary tuberculosis
Agarwal, Alka and Moudgil, K.D. (1988) Enzyme immunoassay based study of IgG and IgM antibody response to antigens of M. tuberculosis (H37 Rv) in patients with pulmonary tuberculosis. Indian Journal of Tuberculosis, 35 (1). pp. 12-16.
Full text available as:
Enzyme immunoassays based on three different antigenic preparations of M. tuberculosis (H37 Rv), i.e. intact cells (1C), sonicate supernatant (SS) and purified protein derivative (PPD), were developed. The assays were used for titration of IgG or IgM antibodies in sera of patients with active pulmonary tuberculosis and normal healthy individual. The tuberculosis sera showed predominantly IgG type of response against 1C, SS and PPD, and the mean IgG reactivity against these antigens was 1,45, 13 and 9 fold higher respectively, as compared to normal controls. On the basis of IgG reactivity, SS and PPD could differentiate 80 7 and 94.73 percent of tuberculosis patients respectively, from normal controls. The IgM response of tuberculosis patients was relatively much less as compared to IgG response, and had no discriminatory significance.
Archive Staff Only: edit this record