Enzyme immunoassay based study of IgG and IgM antibody response to antigens of M. tuberculosis (H37 Rv) in patients with pulmonary tuberculosis

Agarwal, Alka and Moudgil, K.D. (1988) Enzyme immunoassay based study of IgG and IgM antibody response to antigens of M. tuberculosis (H37 Rv) in patients with pulmonary tuberculosis. Indian Journal of Tuberculosis, 35 (1). pp. 12-16.

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Abstract

Enzyme immunoassays based on three different antigenic preparations of M. tuberculosis (H37 Rv), i.e. intact cells (1C), sonicate supernatant (SS) and purified protein derivative (PPD), were developed. The assays were used for titration of IgG or IgM antibodies in sera of patients with active pulmonary tuberculosis and normal healthy individual. The tuberculosis sera showed predominantly IgG type of response against 1C, SS and PPD, and the mean IgG reactivity against these antigens was 1,45, 13 and 9 fold higher respectively, as compared to normal controls. On the basis of IgG reactivity, SS and PPD could differentiate 80 7 and 94.73 percent of tuberculosis patients respectively, from normal controls. The IgM response of tuberculosis patients was relatively much less as compared to IgG response, and had no discriminatory significance.

EPrint Type:Article
Uncontrolled Keywords:Enzyme Immunoassays, Pulmonary Tuberculosis, IgG, IgM, Antigens, Antibodies, Serum, Mycobacterium tuberculosis, Patients, ELISA
Subjects:Immunologic and Biological Factors > Immunologic Factors > Antibodies
Investigative Techniques > Immunologic Techniques
Hemic and Immune Systems > Blood
Persons > Patients
Amino Acids, Peptides, and Proteins > Proteins > Blood Proteins
Bacteria > Gram-Positive Bacteria > Actinobacteria > Actinomycetales > Mycobacteriaceae > Mycobacterium > Mycobacterium tuberculosis
Immunologic and Biological Factors > Immunologic Factors > Antigens > Antigens, Bacterial
Respiratory Tract Diseases > Lung Diseases > Tuberculosis, Pulmonary
-Journal Repositories > Indian Journal of Tuberculosis
Investigative Techniques > Molecular Probe Techniques
Diagnosis > Laboratory Techniques and Procedures
ID Code:3128
Deposited By:Mrs Naina Pandita
Deposited On:03 November 2008

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