Prolactin gene polymorphism and its association with economic traits in Kankrej cattle
Sacravarty, G.; Vadodaria, V.P.; Joshi, C.G.; Brahmkshtri, B.P.; Shah, R.R. and Solanki, J.V. (2008) Prolactin gene polymorphism and its association with economic traits in Kankrej cattle. Indian Journal of Dairy Science, 61 (4). pp. 273-276.
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Polymorphism in 156 bp prolactin gene exon was detected in Kankrej cattle using PCR-RFLP and PCR-SSCP techniques. The frequencies for the genotypes AA, AB and BB were 0.333, 0.352 and 0.315 respectively and the allelic frequency is for A/B alleles were 0.509/0.491. The band patterns obtained in PCR-RFLP technique matched perfectly with the band pattern obtained in PCR-SSCP technique for the three genotypes. Correlation of PRL-Rsa I genotypes with economic traits viz:age at first calving, lactation yield, service period and number of inseminations per conception was done. It indicated that only age at first calving was found to vary significantly between the genotypes with AA and AB cows showing reduced age at first calving than BB cows.
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