Antibacterial activity of culture extracts of Penicillium chrysogenum PCL501: effects of carbon sources

Onyegeme-Okerenta, B.M; Chinedu, S.N.; Okafor, U.A. and Okochi, V.I. (2009) Antibacterial activity of culture extracts of Penicillium chrysogenum PCL501: effects of carbon sources. Online Journal of Health And Allied Sciences, 8 (1). ISSN 0972-5997

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Penicillium chrysogenum PCL501 produced β-lactam antibiotics when fermented with different agro-wastes: cassava shavings, corncob, sawdust and sugarcane pulp. In vitro antibacterial activity of the culture extracts was tested against four clinical bacterial isolates, namely, Bacillus subtilis, Escherichia coli, Klebsiella pneumoniae and Pseudomonas aeruginosa. All the culture extracts and standard drug (commercial Benzyl Penicillin) inhibited the growth B. subtilis and E. coli; the potency varied with carbon source. Antibacterial activity of extracts from cultures containing cassava shavings and sugarcane pulp was comparable with that of the standard drug. The MIC against the susceptible organisms was 0.20mg/ml for the standard drug and ranged from 0.40 to 1.50mg/ml for the culture extracts. Neither the culture extracts nor the standard drug inhibited K. pneumoniae and P. aeruginosa; the bacterial strains produced β-lactamase enzymes. Cassava shavings and sugarcane pulp are indicated as suitable cheap carbon sources for the production of antibiotics by Penicillium chrysogenum PCL501.

EPrint Type:Article
Uncontrolled Keywords:Agrowastes, Penicillium chrysogenum PCL501, Antibiotic production, Phenyl penicillin, Antibacterial activity, β-lactamase, Escherichia coli, Klebsiella pneumoniae, Pseudomonas aeruginosa, Benzyl Penicillin
Subjects:Heterocyclic Compounds > Heterocyclic Compounds, 2-Ring > Bicyclo Compounds, Heterocyclic
Heterocyclic Compounds > Heterocyclic Compounds, 1-Ring > Lactams
Bacteria > Proteobacteria
Chemical Actions and Uses > Pharmacologic Actions > Therapeutic Uses > Anti-Infective Agents > Anti-Bacterial Agents
Heterocyclic Compounds > Heterocyclic Compounds, Bridged-Ring > Bicyclo Compounds, Heterocyclic
Enzymes and Coenzymes > Enzymes > Hydrolases
Fungi > Mitosporic Fungi
Inorganic Chemicals > Sulfur Compounds
Bacteria > Gram-Negative Bacteria > Gram-Negative Aerobic Bacteria > Gram-Negative Aerobic Rods and Cocci > Pseudomonadaceae > Pseudomonas
Organic Chemicals > Sulfur Compounds
-Journal Repositories > Online Journal of Health and Allied Sciences
Organic Chemicals > Amides > Lactams
Bacteria > Gram-Negative Bacteria
Technology, Industry, and Agriculture > Agriculture
Inorganic Chemicals > Elements > Carbon
ID Code:3280
Deposited By:Dr Srinivas Kakkilaya
Deposited On:22 June 2009

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