Genetic characterization and bottleneck studies in Kathiawari horse breed of India
Koringa, P.G.; Joshi, C.G.; Solanki, J.V. and Rank, D.N. (2008) Genetic characterization and bottleneck studies in Kathiawari horse breed of India. Haryana Veterinarians, 47. pp. 77-83.
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To assist the conservation efforts and breeding strategies, the first genetic characterization of the Kathiawari breed of horse was undertaken and 24 microsatellite markers were used to genotype 50 unrelated Kathiawari horses. The observed heterozygosity for the Kathiawari breed was 0.6509 ± 0.1874 with the expected heterozygosity of 0.6448 ± 0.1544. The average number of alleles per locus was 5.166 ± 1.493 while polymorphism information content was 0.591. Wright’s fixation index, FIS (0.1155 + 0.0252) indicated high level of heterozygote deficiency which suggested a high level of inbreeding. This basic study indicated the existence of substantial genetic diversity in the Kathiawari horse population. Significant genotypic linkage disequilibrium was detected across the population, suggesting evidence of linkage between loci. A normal ‘L’ shaped distribution of mode–shift test, non-significant heterozygote excess on the basis of different models, as revealed from Sign, Standardized differences and Wilcoxon sign rank tests as well as non-significant M ratio value suggested that there was no recent bottleneck in the existing Kathiawari breed population, which is important information for equine breeders. This study also revealed that the Kathiawari, a Indian horse breed can be differentiated from some other exotic breeds of horses on the basis of these microsatellite primers.
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