Defensin gene polymorphism analysis in bovine intramammary infections in cattle and buffaloes of Gujarat
Verma, Amit Kumar; Joshi, C.G.; Koringa, P.G. and Nauriyal, D.S. (2009) Defensin gene polymorphism analysis in bovine intramammary infections in cattle and buffaloes of Gujarat. The Indian Journal of Animal Sciences, 79 (3). pp. 300-302. ISSN 0367-8318
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A study was carried out to identify polymorphism at defensin gene loci, resposible for expression of defensin secretion against infection of mammary gland. For this, blood samples were collected from Kankrej, Triple cross, Holstein Friesian and buffaloes. From each category of cows and buffaloes, samples were collected each from animals with clinical mastitis, subclinical mastitis and healthy udder. PCRRFLP was carried out from the extracted genomic DNA. Four polymorphism patterns of combined defensin genotypes CDG were obtained. No significant correlation was obsorved between these groups of animals. Similarly, no breed correaltion was recorded between animals with clinical and subclinical mastitis and healhty udder. It may be concluded that the indigenous Kankrej cattle and buffaloes located in Gujarat are genetically closer to each other than exotic and crossbred animals.
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