Evaluation of a rapid molecular method for detection of Listeria monocytogenes directly from broth culture

Mathakiya, R.A.; Roy, A.; Nandasana, K.N.; Koringa, P.G. and Joshi, C.G. (2009) Evaluation of a rapid molecular method for detection of Listeria monocytogenes directly from broth culture. Veterinary World, 2 (5). pp. 177-178. ISSN 0972-8988

Full text available as:

PDF - Requires Adobe Acrobat Reader or other PDF viewer.
245 Kb

Abstract

The present study was carried out to know the lowest detection limit of Listeria monocytogenes by PCR. The quantification of organisms was done by CFU counting from ten fold serial dilution and PCR amplification of inlA gene fragment was performed from each dilution. The PCR could detect as low as 20 organisms indicating the lowest PCR detection limit. Thus, lowest number of L. monocytogenes detectable by PCR is a low-cost and rapid procedure that can be appropriated for the detection in real time of low L. monocytogenes levels in naturally contaminated food and is suitable to implement in the food industry.

EPrint Type:Article
Additional Information:PCR based detection - CFU - Listeria monocytogenes
Uncontrolled Keywords:Quantification, CFU counting, Serial dilution, PCR, Low-cost, Food industry
Subjects:-Institutional Repositories > College of Veterinary Science and Animal Husbandry (Anand Agricultural University)
Investigative Techniques > Clinical Laboratory Techniques > Culture Techniques
Bacteria > Gram-Positive Bacteria
Equipment and Supplies > Culture Media
Diagnosis > Early Diagnosis
Bacteria > Gram-Negative Bacteria
Biological Sciences > Biotechnology
Investigative Techniques > Molecular Probe Techniques
Diagnosis > Laboratory Techniques and Procedures
ID Code:3300
Deposited By:Dr Prakash Koringa
Deposited On:20 July 2009

Archive Staff Only: edit this record