Evaluation of group specific nested PCR for detection of bluetongue virus

Chauhan, H.C.; Kher, H.N.; Chandel, B.S.; Dadawala, A.I.; Jain, L.; Agrawal, S.M. and Bhadaniya, A. (2009) Evaluation of group specific nested PCR for detection of bluetongue virus. Veterinary World, 2 (5). pp. 179-182.

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Abstract

During the present study RT-PCR and nested PCR was evaluated for the detection of BTV in blood samples collected from suspected cases of bluetongue. Due to conserved nature, NS1 gene was targeted for the development of partial length and nested PCR assay. The partial length RT-PCR and nested PCR assays yielded a specific PCR product of expected 274 bp and 101 bp sizes, respectively. In, the present study out of 68 blood samples processed for BTV detection, 2 samples were found positive for BTV genome by RT-PCR as well as in nested PCR. The described BTV PCR based assay provides a valuable tool to study the epidemiology of BTV infection in susceptible domestic livestock.

EPrint Type:Article
Uncontrolled Keywords:Ruminant, Mortality, Genome, PCR assays, Epidemiology, Economic, Bluetongue
Subjects:Environment and Public Health > Public Health > Epidemiologic Measurements > Demography > Vital Statistics > Mortality
-Journal Repositories > Veterinary World
Social Sciences > Economics
Health Care Economics and Organizations > Economics
Investigative Techniques > Epidemiologic Methods > Data Collection > Vital Statistics > Mortality
Animal Diseases > Sheep Diseases > Bluetongue
Animals > Chordata > Vertebrates > Mammals > Artiodactyla > Ruminants
Viruses > RNA Viruses
Investigative Techniques > Genetic Techniques
Diagnosis > Laboratory Techniques and Procedures
ID Code:3307
Deposited By:Dr Anjum Sherasiya
Deposited On:19 August 2009

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