Identification and characterisation of Mycobacteria other than TB by protein finger printing

Jain, Naresh K.; Kachroo, Bharat B.; Prasad, Govind; Hanifa, M. and Mandal, A. (1991) Identification and characterisation of Mycobacteria other than TB by protein finger printing. Indian Journal of Tuberculosis, 38 (2). pp. 91-94.

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Abstract

Whole cell extracts of M gordonae and M tuberculosis (clinical isolate and H37Rv) were prepared arid electrophbresed on 10% poly-acrylarntcle gels. Staining of the gels shows many prominent bands of M, gordanae distinct from that of M, tuberculosis. The douhle diffusion pattern shows cross-reacting and distinct bands among different species of mycobacteria. The immunoblot of M gordonae with hyperimmune serum shows three distinct bands of 24, 26 and 58 KDa, while 55 KDa antigen cross reacts with M tuberculosis serum. The data show that there is a good possibility of developing a specific and rapid system for identification and characterization of non-tuberculous mycobacteria by Protein Finger Printing technique.

EPrint Type:Article
Uncontrolled Keywords:Tuberculosis, Finger Printing Mycobacteria species, Mycobacteria tuberculosis, Mycobacteria gordonae, Immublotting, Culture, Serum, Antigens
Subjects:Bacterial Infections and Mycoses > Bacterial Infections > Gram-Positive Bacterial Infections > Actinomycetales Infections > Mycobacterium Infections > Tuberculosis
Equipment and Supplies > Culture Media
Bacteria > Gram-Positive Bacteria > Actinobacteria > Actinomycetales > Mycobacteriaceae > Mycobacterium > Mycobacterium tuberculosis
Immunologic and Biological Factors > Immunologic Factors > Antigens > Antigens, Bacterial
Bacterial Infections and Mycoses > Bacterial Infections > Gram-Positive Bacterial Infections > Actinomycetales Infections > Mycobacterium Infections
Investigative Techniques > Molecular Probe Techniques
Investigative Techniques > Clinical Laboratory Techniques > Culture Techniques
Investigative Techniques > Immunologic Techniques
Investigative Techniques > Chemistry, Analytical
-Journal Repositories > Indian Journal of Tuberculosis
ID Code:3385
Deposited By:Mrs Naina Pandita
Deposited On:06 October 2009

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