PCR based detection of virulence associated genes of Staphylococcus aureus from bovine clinical and subclinical mastitis

Bhanderi, B.B.; Roy, A.; Yadav, M.M. and Joshi, C.G. (2009) PCR based detection of virulence associated genes of Staphylococcus aureus from bovine clinical and subclinical mastitis. The Royal Veterinary Journal of India, 5 (I & II). pp. 20-26. ISSN 0973-0443

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Abstract

Total 43 S. aureus were isolated from 121 clinical and subclinical mastitis cases of cow and buffloes of Gujarat state, India. All the S. aureus were isolated on the basis of morphological, cultural and biochemical. All the 43 S. aureus were identified by PCR method using species specific oligonulceotide primers that encodes gene 23 s rRNA of S. aureus which yielded an amplification product of 1250 bp. PCR base detection was carried out for virulence associated genes of S. aureus namely clumping factor (clfA), the Ig G binding region of the protein A (spa), the coagulase (coa) and the methicillin (mec) resistance. 42 isolate yieled an amplicon of 980 bp for clfA gene with no size polymorphism. The PCR amplification for the gene encloding the Ig G region of protein A yielded a product size of 920 bp for 38 isolates while 4 isolate revelaed a product size 730 bp indicating polymorphims of this gene. Gene for the coagulase production was detected in 23 out of 43 isolates with ampified product of 670 bp (16 isolates) and 850 bp (7 isolates) indicating polymorphims of this gene. While methicillin resisntance gene of S. aureus isolate did not generate any amplified product.

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