Cloning and sequencing of the leptin gene in gir cattle and Mehsana buffalo
Jhala, N.B.; Rank, D.N.; Vataliya, P.H.; Joshi, C.G.; Bhong, C.D.; Mehta, H.H. and Patil, A.V. (2009) Cloning and sequencing of the leptin gene in gir cattle and Mehsana buffalo. Buffalo Bulletin, 28 (1). pp. 29-33. ISSN 0125-6726
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The leptin gene is considered as a potential candidate gene influencing different production traits in dairy cattle, for example, milk performance and reproduction. Mutations in its sequences may alter animal performance as well as their breeding values. PCR products of Gir cattle and Mehsana buffalo were purified and ligated to pTZ57R/T vector supplied with the InsT/A cloneTM cloning kit. The cloned PCR products (695 bp) of leptin gene exon-3 were sequenced. The consensus sequence obtained was 508 bp in Gir cattle and 517 bp in Mehsana buffalo. There was sequence variation between cattle and buffalo at 11 positions. The sequence obtained from Gir showed two point mutations (at positions 264 and 318), while the sequence obtained from Mehsana buffalo showed three point mutations (at positions 42, 44 and 250) as compared to published sequence.
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