Detection of bovine herpesvirus (BHV-1) infection in semen of breeding bulls of Gujarat by a direct fluorescence test
Jain, Lata; Kanani, A.N.; Patel, T.J.; Purohit, J.H.; Jhala, M.K.; Joshi, C.G.; Chandel, B.S. and Chauhan, H.C. (2008) Detection of bovine herpesvirus (BHV-1) infection in semen of breeding bulls of Gujarat by a direct fluorescence test. Buffalo Bulletin, 27 (2). pp. 202-206. ISSN 0125-6726
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Bovine herpesvirus 1 (BHV-1), the causative agent of infectious bovine rhinotracheitis (IBR), is considered to be the most common viral pathogen found in bovine semen. BHV-1 is associated with several clinical conditions including infectious bovine rhinotracheitis, infectious pustular vulvovaginitis, balanoposthitis, conjunctivitis and generalized disease in newborn calves causing great economic loss to the livestock industry. The present study was undertaken to detect the presence of viral antigen by direct immunofluorescence in semen of breeding bulls of five different semen collection centres of Gujarat. A total of 101 semen samples from cattle and buffalo breeding bulls were tested for BHV-1 antigen using a direct immunofluorescence kit made available by VMRD, Inc. Pullman, USA., and green fluorescence was observed in 33 (32.67%) samples. Out of 49 cattle bulls and 52 buffalo bulls, 16 and 17 bulls, respectively, were found to be positive. This shows an equal distribution of BHV-1 antigen in both the species. Finally, the study revealed presence of BHV-1 in the semen of breeding bulls of Gujarat. Thus, under the Sexual Health Control Programme, proper measures must be taken at the State level for controlling BHV-1 infection. All breeding bulls must be tested periodically for detection of both BHV-1 antibody in serum and the presence of BHV-1 in semen. The bulls must be free from BHV-1 infection prior to use.
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