Evaluation of PCR and Indirect enzyme linked immunosorbent essay on milk samples for diagnosis of brucellosis in bovines

Patel, T.J.; Kanani, A.N.; Jain, Lata; Joshi, C.G. and Purohit, J.H. (2008) Evaluation of PCR and Indirect enzyme linked immunosorbent essay on milk samples for diagnosis of brucellosis in bovines. Buffalo Bulletin, 27 (2). pp. 207-211. ISSN 0125-6726

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Abstract

Polymerase chain reaction (PCR) was used for detection of Brucella DNA in bovine milk. A genus specific primer pair which amplified a 223 bp fragment of gene encoding a 31-kDa Brucella abortus antigen was used in assay. The efficacy of the PCR was evaluated by comparing with the result of milk-ELISA for the diagnosis of Brucella infection in bovines. The milk-ELISA resulted in more positive samples as compared to PCR, since it detected Brucella antibodies in 15 milk samples out of 53 bovines while Brucella DNA detected the antibodies in nine out of 53 milk samples. The results suggest that ELISA is a better screening test than PCR, and their simultaneous application could be more useful than one test alone for a rapid screening of brucellosis in bovines.

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