Blood biochemical profiles at different stages of gestation in triple cross-bred (K x J x HF) cattle
Padodara, R.J. (2004) Blood biochemical profiles at different stages of gestation in triple cross-bred (K x J x HF) cattle. Masters thesis, Anand Agricultural University.
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A study was conducted on fourty four triple cross-bred (½ Kankarej X ¼ Jersey X ¼ Holstein Friesian) pregnant heifers (20) and cows (24) at Livestock Research Station, Veterinary College, Anand Agricultural University, Anand with the objective to know various blood biochemical profiles at different stages of gestation. The blood was collected from these animals through jugular venipuncture once a day on days 90,150, 210 and 275 of gestation. The blood samples were analysed for various haematological, biochemical and hormonal parameters using standard laboratory procedure. Albumin and Triglyceride were estimated by kit (Crest Biosystems, Goa) using semi auto-analyser instrument. Trace elements were estimated by using Atomic absorption spectrophotometer (ECIL, AAS4141). Hormones viz. T3, T4 and Insulin were estimated by RIA method using kits of BARC, Mumbai, while Progesterone and Estradiol-17β were analysed by using kits of ImmunoTech, France. Haematological and PCV both were found decreased significantly (p<0.01) as the pregnancy advanced. Hormonal parameters of serum T3 and Insulin differed non-significantly but serum T4, Progesterone and Estradiol showed significant (p<0.01) difference during various stages of gestation. Biochemical parameters such as blood glucose, serum cholesterol and triglyceride were found decreased significantly (p<0.01) as the pregnancy advanced. Serum albumin also was found decreased significant (p<0.05) with increased gestation period. Serum total protein, globulin, and albumin: globulin ratio did not differ due to stage of gestation. Serum calcium, inorganic phosphorus and their ratios were found significantly (p<0.01) decreased from day 90 to 275 of gestation. Trace elements manganese and cobalt were found to show increasing (p<0.01) trend but no significant difference was found in levels of serum iron, copper and zinc. The serum electrolytes, sodium and potassium showed increasing trend during different stages of gestation. But serum enzyme, Acetyl Cholinesterase showed no definite trend during different stages of gestation.
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