Evaluation of sputum smears prepared by different methods
Naganathan, N.; Ganapathy, K.T. and Rajalakshmi, R. (1979) Evaluation of sputum smears prepared by different methods. Indian Journal of Medical Research, 69. pp. 893-900.
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A study was conducted to compare the sensitivity of 4 methods of sputum smear preparation viz., direct smear prepared (i) blindly without making any selection of portions of sputum specimen, (ii) from portions of sputum material likely to contain the bacilli, (iii) after mixing up the sputum specimens thoroughly, and (iv) a smear prepared from centrifuged deposit after homogenisation of sputum with sodium hydroxide and concentration by centrifugation. There was practically no difference in the number of positives obtained from different methods. Centrifuged deposit smears proved to be in no way better than the direct smears. The differences in the methods lay only in the classification of positive smear as of a low or high grade.
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