Ziehl-Neelsen staining of sputum smears avoiding the step of decolourisation with alchol

Chauhan, M.M.; Mahadev, B.; Chandrasekaran, Sujatha; Rajalakshmi, R. and Chaudhuri, K. (1990) Ziehl-Neelsen staining of sputum smears avoiding the step of decolourisation with alchol. NTI Newsletter, 26 (1). pp. 21-24.

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There has been a lot of constraints for the availability of alcohol in Peripheral Health Institutions (PHIs) and this is the reason often given for not doing the smear examination in the PHIs. In this study, 419 smears were prepared in duplicate and one set was stained by the conventional Ziehl-Neelsen (ZN) procedure (A method). In the other set, decolourisation with alcohol was omitted (B method). The analysis of the results show that 14.6% of the positive smears picked up by the conventional ZN method was missed by B method where the step of decolourisation with alcohol was omitted. The present study was undertaken to evaluate the ability to pick up cases by ZN staining omitting the step of decolourisation with alcohol.

EPrint Type:Article
Additional Information:Abstract prepared by OpenMED staff
Uncontrolled Keywords:Ziehl-Neelsen procedure, Sputum, Pulmonary Tuberculosis, Smear studies
Subjects:Fluids and Secretions > Bodily Secretions > Sputum
Bacterial Infections and Mycoses > Bacterial Infections > Gram-Positive Bacterial Infections > Actinomycetales Infections > Mycobacterium Infections > Tuberculosis
-Institutional Repositories > National Tuberculosis Institute
Respiratory Tract Diseases > Respiratory Tract Infections > Tuberculosis, Pulmonary
Investigative Techniques > Clinical Laboratory Techniques > Culture Techniques
Organic Chemicals > Alcohols
Respiratory Tract Diseases > Lung Diseases > Tuberculosis, Pulmonary
ID Code:458
Deposited By:Librarian Sudha S Murthy
Deposited On:29 July 2005

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