Ziehl-Neelsen staining of sputum smears avoiding the step of decolourisation with alchol
Chauhan, M.M.; Mahadev, B.; Chandrasekaran, Sujatha; Rajalakshmi, R. and Chaudhuri, K. (1990) Ziehl-Neelsen staining of sputum smears avoiding the step of decolourisation with alchol. NTI Newsletter, 26 (1). pp. 21-24.
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There has been a lot of constraints for the availability of alcohol in Peripheral Health Institutions (PHIs) and this is the reason often given for not doing the smear examination in the PHIs. In this study, 419 smears were prepared in duplicate and one set was stained by the conventional Ziehl-Neelsen (ZN) procedure (A method). In the other set, decolourisation with alcohol was omitted (B method). The analysis of the results show that 14.6% of the positive smears picked up by the conventional ZN method was missed by B method where the step of decolourisation with alcohol was omitted. The present study was undertaken to evaluate the ability to pick up cases by ZN staining omitting the step of decolourisation with alcohol.
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