Assessment of trisodium phosphate for storage and isolation of mycrobacteria in single step culture method

Chauhan, M.M.; Mahadev, B; Balasangameshwara, V.H. and Srikantaramu, N. (1999) Assessment of trisodium phosphate for storage and isolation of mycrobacteria in single step culture method. Indian Journal of Tuberculosis, 46. pp. 29-36.

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Three hundred smear positive and two hundred and ninety-nine smear negative sputum specimens were allocated by random to Trisodium Phosphate (TSP)/ Modified Petroff’s (MP) methods, stored up to eight days and then processed, in order to assess the merits of TSP as a medium for transportation and isolation of mycobacteria. In the TSP method, the overall culture positivity was 51% and contamination rate was 0.7%. The results among smear positive specimens in respect of culture positivity and contamination rates were 90.1% and 0.6% respectively. Among smear negative specimens, the contamination rate was 0.7%. These values were significantly better when compared with the Modified Petroff’s method which served as the control. Another important finding of the study was that sputum positive specimens could be stored for up to 8 days in the TSP solution, with significantly less killing of mycobacteria and a lower contamination rate. However, in respect of smear negative specimens the TSP method compared with the MP, method yielded fewer positive cultures when specimens were stored for more than 4 days.

EPrint Type:Article
Uncontrolled Keywords:Single-step culture, TSP for culture, Storage of sputum, Transport medium, Trisodium Phosphate
Subjects:Fluids and Secretions > Bodily Secretions > Sputum
Bacterial Infections and Mycoses > Bacterial Infections > Gram-Positive Bacterial Infections > Actinomycetales Infections > Mycobacterium Infections > Tuberculosis
-Institutional Repositories > National Tuberculosis Institute
Equipment and Supplies > Culture Media
Respiratory Tract Diseases > Respiratory Tract Infections > Tuberculosis, Pulmonary
Respiratory Tract Diseases > Lung Diseases > Tuberculosis, Pulmonary
-Journal Repositories > Indian Journal of Tuberculosis
Inorganic Chemicals > Sodium Compounds
ID Code:468
Deposited By:Librarian Sudha S Murthy
Deposited On:29 July 2005

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