Assessment of trisodium phosphate for storage and isolation of mycrobacteria in single step culture method
Chauhan, M.M.; Mahadev, B; Balasangameshwara, V.H. and Srikantaramu, N. (1999) Assessment of trisodium phosphate for storage and isolation of mycrobacteria in single step culture method. Indian Journal of Tuberculosis, 46. pp. 29-36.
Full text available as:
Three hundred smear positive and two hundred and ninety-nine smear negative sputum specimens were allocated by random to Trisodium Phosphate (TSP)/ Modified Petroff’s (MP) methods, stored up to eight days and then processed, in order to assess the merits of TSP as a medium for transportation and isolation of mycobacteria. In the TSP method, the overall culture positivity was 51% and contamination rate was 0.7%. The results among smear positive specimens in respect of culture positivity and contamination rates were 90.1% and 0.6% respectively. Among smear negative specimens, the contamination rate was 0.7%. These values were significantly better when compared with the Modified Petroff’s method which served as the control. Another important finding of the study was that sputum positive specimens could be stored for up to 8 days in the TSP solution, with significantly less killing of mycobacteria and a lower contamination rate. However, in respect of smear negative specimens the TSP method compared with the MP, method yielded fewer positive cultures when specimens were stored for more than 4 days.
Archive Staff Only: edit this record