Salmonella typhimurium grown in iron-rich media, inactivated with ferric chloride and adjuvanted with homologous bacterial DNA is potent and efficacious vaccine in mice.

Kumar, Devender and Singh, Ajit (2005) Salmonella typhimurium grown in iron-rich media, inactivated with ferric chloride and adjuvanted with homologous bacterial DNA is potent and efficacious vaccine in mice. Vaccine, 24 (Articles in Press (Advanced online publication)). ISSN 0264-410X

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Abstract

The present study describes our attempt to construct a novel vaccine formulation that affords full protection against murine typhoid under experimental conditions. Ferric chloride, 100 mM, as inactivating agent, bacterial growth under iron-rich conditions and homologous bacterial DNA as adjuvant were used for construction of the experimental Salmonella typhimurium vaccine. The vaccine inoculated twice at 2 weeks interval in Swiss albino mice elicited statistically significant IgG levels when compared with non-adjuvanted and other control groups. All the mice inoculated with the novel vaccine withstood challenge with 50 LD50 dose of S. typhimurium strain St585. No significant safety problems were found in mice.

EPrint Type:Article
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Uncontrolled Keywords: Iron-inactivated vaccine; Homologous bacterial DNA adjuvant; Salmonella typhimurium
Subjects:Animal Diseases > Salmonella Infections, Animal
Nucleic Acids, Nucleotides, and Nucleosides > Nucleic Acids > DNA
Immunologic and Biological Factors > Biological Products > Vaccines
Immunologic and Biological Factors > Immunologic Factors > Adjuvants, Immunologic
ID Code:533
Deposited By:Dr Devender Kumar
Deposited On:02 August 2005

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