Comparison between rapid colorimetric mycobacterial isolation and susceptibility testing method and conventional method using LJ medium

Mahadev, B.; Srikantaramu, N.; James, P.; Mathew, P.G. and Bhagirathi, R. (2001) Comparison between rapid colorimetric mycobacterial isolation and susceptibility testing method and conventional method using LJ medium. Indian Journal of Tuberculosis, 48. pp. 129-134.

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Abstract

The objective of the study was to evaluate mycobacterial isolation rates, Mean Detection Time(MDT)and reliability of susceptibility as well as identification procedures in regard to clinical specimens by colorimetric and conventional methods. A total of 205 clinical specimens were processed by modified Petroff’s method and then inoculated into MB/BacT-240 system bottles and on LJ medium slopes. A total of 101 isolates were detected by both the methods: the recovery rate was 57.1% (117/205) by the colorimetric method and 55.1% (113/ 205) by the conventional method. Contamination rates were 1% & 6.8% respectively. The MDT including susceptibility testing time was 28 and 52 days respectively. Highly significant difference was observed between the two sample means in the colorimetric and conventional methods. Colorimetric method enables rapid detection and drug susceptibility testing, making it possible to get the results four weeks earlier compared to the conventional method. But, the Para-nitro Benzoic Acid (PNB) test by the colorimctric method needs standardization.

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