Serodiagnosis of pulmonary tuberculosis and evaluation of two ELISA kits
Chandrasekaran, Sujatha; Chauhan, M.M. and Parimala, N. (1996) Serodiagnosis of pulmonary tuberculosis and evaluation of two ELISA kits. Indian Journal of Tuberculosis, 43. pp. 159-162.
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An investigation was carried out to assess the value of ELISA test in the serodiagnosis of pulmonary tuberculosis and the effectivenessof two ELISA test kits, using A60 and KP90 antigens respectively. Sputum culture was performed to, confirm the diagnosis. Serum dilution of 1:100 was used and Ig Gant! bodies to A60 and Ig A antibodies to KP90 were looked for. A total of 317 patients were studied. The cut-off point (mean+SD)was taken from healthy controis. The Sensitivity, specificity and accuracy of KP90 ELISA were 49.7%, $4.0% and 61.5 % and those of A60 ELISA were 48.3 %, 92.0% and 71.3% respectively. It is considered that ln h igh prevalence conditions, serological tests for tuberculosis based on antibody detection should be used and interpreted with extreme caution. Their results should definitely score over sputum micros 1 copy results ln order. to be widely applied.
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