Some research in isolation of tubercle bacilli
Barton, R.M. (1956) Some research in isolation of tubercle bacilli. Indian Journal of Tuberculosis, 3 (2). pp. 36-42.
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(1) A brief account is presented of a number of small researches related to the isolation of tubercle bacilli. (2) With the growing volume of culture work in tuberculosis laboratories there is a demand for simplification of technique. (3) A comparison of simultaneous cultures of gastric contents and a laryngeal swab show that there would be a loss in positive findings if the cultures of gastric contents were omitted. (4) Sputum swab culture is compared with homogenized sputum culture in 837 specimens and superior results are obtained with homogenized sputum. (5) Under certain conditions such as may be found in field work delay in culturing laryngeal swab seems possible, but further research is still needed at higher atmospheric temperature. It is not possible to reduce the number of laryngeal swabs from two to one without loss of positive results. (6) The effect of light on culture work has been studied over one year and in general appears to have no harmful effect. (7) Short storage of Jensen-Lowenstein medium at room temperature does not seem to effect the value of the medium.
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