A simple pre-treatment technique for sputum comprising the use of a combination of pancreatin and cetavlon for the routine cultivation of tubercle bacilli

Sikand, B.K. and Ranga Rao, A. (1958) A simple pre-treatment technique for sputum comprising the use of a combination of pancreatin and cetavlon for the routine cultivation of tubercle bacilli. Indian Journal of Tuberculosis, 5 (2). pp. 76-86.

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Abstract

1. A new technique for the cultivation of tubercle bacilli from routine sputum specimens using a combination of pancreatin-cetavlon (PC) mixture in a Phosphate Buffer of pH 8.0 is described. 2. The pancreatin-cetavlon (PC) mixture was used for the pre-treatment of 1033 routine sputum specimens for the primary isolation of tubercle bacilli. For comparison, all the sputum specimens were also subjected to the 4 % sodium hydroxide pre-treatment technique. 3. The pancreatin-cetavlon (PC) pre-treatment technique for sputum was found to be as efficient and sensitive as the 4% sodium hydroxide pre-treatment technique in the detection of tubercle bacilli. The (PC) technique also gave more rapid positive culture results than the latter. 4. The pancreatin-cetavlon (PC) pre-treatment technique for sputum is recom-” mended for use in the routine isolation of tubercle bacilli, as a reliable, simple and safe one, since it avoids a costly “high-speed centrifuge”, too many reagents and procedures which involve hazards to personnel and the laboratory. 5. Laboratories and clinics so far not doing culture work can now use the (PC) technique in addition to their direct smear examinations with great advantage to their patients

EPrint Type:Article
Uncontrolled Keywords:Pre-Treatment technique, Sputum, Pancreatin, Cetavlon, Tubercle Bacilli, Culture
Subjects:Fluids and Secretions > Bodily Secretions > Sputum
Enzymes and Coenzymes > Enzymes > Hydrolases
Equipment and Supplies > Culture Media
Bacteria > Gram-Positive Bacteria > Actinobacteria > Actinomycetales > Mycobacteriaceae > Mycobacterium > Mycobacterium tuberculosis
Complex Mixtures > Tissue Extracts > Pancreatic Extracts
Chemical Actions and Uses > Specialty Uses of Chemicals > Laboratory Chemicals
Investigative Techniques > Clinical Laboratory Techniques > Culture Techniques
-Journal Repositories > Indian Journal of Tuberculosis
ID Code:847
Deposited By:Mrs Naina Pandita
Deposited On:02 November 2005

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