A cold staining method for tubercle bacilli using chloroform
Padmanabha Rao, K.; Naganathan, N. and Nair, S.S. (1966) A cold staining method for tubercle bacilli using chloroform. Indian Journal of Tuberculosis, 14 (1). pp. 4-9.
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A cold staining (CS) method using chloroform was compared with the conventional Ziehl- Neelsen (ZN)and fluorescence microscopy (FM) for evidence of tubercle bacilli in sputum smears. The reliability of these methods was judged on the basis of culture results and agreement among themselves. The CS method was found to be as efficient as the ZN method in detecting different gradings of culture positives. above are sufficiently encouraging to expect better and reliable results by CS method. The section on findings showed that this method was atleast as good as ZN and FM methods and even indicated that it may be better than the other two as it gives less false positives. False positives under the method were significantly lower than by ZN or FM microscopy. Some possible explanations for the relatively less number of false positives under CS method have been discussed. Preparation of stain for cold staining is simple and in the procedure of staining, heat- ing, a much too precise step in ZN method for large scale application, is eliminated. To reduce false positives in FM, further investigation has been suggested employing increased strength of mineral acid for decolorisation.
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