A cold staining method for tubercle bacilli using chloroform

Padmanabha Rao, K.; Naganathan, N. and Nair, S.S. (1966) A cold staining method for tubercle bacilli using chloroform. Indian Journal of Tuberculosis, 14 (1). pp. 4-9.

Full text available as:

PDF - Requires Adobe Acrobat Reader or other PDF viewer.
204 Kb


A cold staining (CS) method using chloroform was compared with the conventional Ziehl- Neelsen (ZN)and fluorescence microscopy (FM) for evidence of tubercle bacilli in sputum smears. The reliability of these methods was judged on the basis of culture results and agreement among themselves. The CS method was found to be as efficient as the ZN method in detecting different gradings of culture positives. above are sufficiently encouraging to expect better and reliable results by CS method. The section on findings showed that this method was atleast as good as ZN and FM methods and even indicated that it may be better than the other two as it gives less false positives. False positives under the method were significantly lower than by ZN or FM microscopy. Some possible explanations for the relatively less number of false positives under CS method have been discussed. Preparation of stain for cold staining is simple and in the procedure of staining, heat- ing, a much too precise step in ZN method for large scale application, is eliminated. To reduce false positives in FM, further investigation has been suggested employing increased strength of mineral acid for decolorisation.

EPrint Type:Article
Uncontrolled Keywords:Cold Staining Method, Tubercle bacilli, Chloroform, Conventional Ziehl-Neelsen (ZN) Method, Sputum Smears
Subjects:Fluids and Secretions > Bodily Secretions > Sputum
Equipment and Supplies > Culture Media
Bacteria > Gram-Positive Bacteria > Actinobacteria > Actinomycetales > Mycobacteriaceae > Mycobacterium > Mycobacterium tuberculosis
Investigative Techniques > Microscopy
Investigative Techniques > Clinical Laboratory Techniques > Culture Techniques
Organic Chemicals > Hydrocarbons > Hydrocarbons, Halogenated
-Journal Repositories > Indian Journal of Tuberculosis
ID Code:999
Deposited By:Mrs Naina Pandita
Deposited On:13 December 2005

Archive Staff Only: edit this record